Impact of Exercise Endurance Training on PurB Gene Expression and Cardiac Function

Introduction: Endurance training has significant effects on the renewal of heart tissue, including myosin heavy chain (MHC) proteins. On the other side, Purine-rich element-binding protein &beta (purB) decreases the &alphaMHC gene expression. The aim of this study was to determine the impact of exercise endurance training on purB gene expression in the heart of Wistar rats. Methods: Fourteen rats have been kept under controlled conditions and after familiarizing with training protocol, they were divided into control groups and experimental groups. The experimental group performed a 10-week treadmill running program for 30 min/day, 5 days/week. 48 hours after the last training session, the rats were anesthetized and the heart and their left ventricle were taken out and purB expression was measured using real time PCR method. All data were analyzed using t test. Results: In this study, the results of M-mode echocardiography showed that endurance training led to cardiac hypertrophy. After endurance training, the heart weight, especially the left ventricular weight significantly increased. The purB gene expression significantly decreased in the left ventricular tissue of endurance-trained rats. Conclusion: The results of this study revealed that endurance training has considerable effects on heart size and purB gene expression. The purB gene also repressed Alpha-MHC gene expression it seems that the changes in heart structure related to Alpha-MHC gene expression.