Immunogenicity of a New HIV-1 DNA Construct in a BALB/c Mouse Model

نویسندگان

  • Farzaneh Pourasgari Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Company, Tehran, Iran
  • Fatemeh Rahbarizadeh Medical Biotechnology, Tarbiat Modares University
  • Hamidreza Korram Khorshid Genetics Research Centre, University of Social Welfare and Rehabilitation Sciences | Reproductive Biotechnology Research Centre, Avicenna Research Institute
  • Haydeh Darabi immunology Pasteur Institute of Iran
  • Kayhan Azadmanesh Virology
  • Mehdi Mahdavi Departments of Immunology
  • Zuhair Mohammad Hassan Departments of Immunology
چکیده مقاله:

Background: Cell mediated immunity, especially cytotoxic T cell responses against HIV-1 infection, plays a critical role in controlling viral replication and disease progres-sion. DNA vaccine is a novel technology which is known to stimulate strong cellular immune responses. Many DNA vaccines have been tested for HIV infection but there is still no effective vaccine against this infection. Construction of a vaccine consisting of multiple conserved and immunogenic epitopes may increase vaccine efficacy. Objective: In the present study, a DNA vaccine candidate constructed from HIV-1 P24-Nef was evaluated and cellular immune responses were assessed in murine BALB/c model. Methods: HIV-1 P24-Nef gene was cloned in pCDNA3.1 expression vector. Mice were immunized with DNA construct and IL-4 and IFN-γ evaluation was per-formed using ELISPOT. Cytotoxicity response was evaluated with Granzyme B ELIS-POT assay and lymphocyte proliferation was evaluated with LTT assay. Results: Analysis of immune responses showed that, compared to control groups, the candidate vaccine induced production of higher levels of both IL-4 and IFN-γ (p

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عنوان ژورنال

دوره 6  شماره 4

صفحات  163- 173

تاریخ انتشار 2009-12-01

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