IL-10 and IL-12 Production in Response to Mycobacterium Tuberculosis Total Lipid Antigens in Multidrug-Resistant Tuberculosis

نویسندگان

  • Ahmad Zavaran Hosseini Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  • Ali Shams Shahemabadi Department of Immunology, Faculty of Medical Sciences, Shaheed Sadughi University of Medical Sciences, Yazd, Iran
  • Majid Pouramiri National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Masih Daneshvari Hospital, Beheshti University of Medical Sciences, Tehran, Iran
  • Majid Rayani National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Masih Daneshvari Hospital, Beheshti University of Medical Sciences, Tehran, Iran
  • Majid Shams National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Masih Daneshvari Hospital, Beheshti University of Medical Sciences, Tehran, Iran
  • Mohammad Reza Masjedi National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Masih Daneshvari Hospital, Beheshti University of Medical Sciences, Tehran, Iran
  • Nasrin Esphandyari National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Masih Daneshvari Hospital, Beheshti University of Medical Sciences, Tehran, Iran
  • Shapour Shaghasempour National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Masih Daneshvari Hospital, Beheshti University of Medical Sciences, Tehran, Iran
چکیده مقاله:

Background: Mycobacterium tuberculosis lipid antigens take part in pathogenicity of the bacterium but the response of monocytes/macrophages to these antigens in tubercu-losis is not well known. Objective: The aim of current investigation was to study the M. tuberculosis lipid antigens in tuberculosis pathogenesis. Methods: In the present study M. tuberculosis lipid antigens were extracted. Monocytes and macrophages from mul-tidrug-resistant tuberculosis (MDR-TB), TB patients, asymptomatic healthy individuals with positive tuberculin skin test positive and healthy individuals with negative tubercu-lin skin test were collected using magnetic cell sorting. The cells were stimulated by M. tuberculosis total lipid antigens and IL-12 and IL-10 in their supernatants were meas-ured by enzyme-linked immunosorbent assay. Results: The IL-12 production by mono-cytes in response to M. tuberculosis total sonicate antigens in the MDR-TB patients did not show a considerable difference with the PPD positive healthy subjects, whereas in the active TB patients, IL-12 levels significantly decreased (p

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il-10 and il-12 production in response to mycobacterium tuberculosis total lipid antigens in multidrug-resistant tuberculosis

background: mycobacterium tuberculosis lipid antigens take part in pathogenicity of the bacterium but the response of monocytes/macrophages to these antigens in tubercu-losis is not well known. objective: the aim of current investigation was to study the m. tuberculosis lipid antigens in tuberculosis pathogenesis. methods: in the present study m. tuberculosis lipid antigens were extracted. mono...

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IL-10 and IL-12 production in response to Mycobacterium tuberculosis total lipid antigens in multidrug resistant tuberculosis.

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عنوان ژورنال

دوره 7  شماره 1

صفحات  57- 63

تاریخ انتشار 2010-03-01

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