Green synthesis of Au-Ag nanoparticles using Mentha piperita and effects of Au-Ag alloy nanoparticles on the growth of Mentha piperita under salinity stress

Salinity stress reduces plant metabolic activity, which reduces plant growth in saline environments. this study aimed to investigate the effect of salinity stress as one of the limiting factors of plant growth and the effect of nanoparticles on stress conditions as one of the auxiliary factors in plant control against stressful conditions by studying physiological factors such as soluble sugars, chlorophyll, carotenoids, Proline, anthocyanins, antioxidant enzymes have been investigated. For this purpose, a factorial experiment was conducted in a completely randomized design with three replications. The first factor is tension Salinity with different concentrations of salt, including zero, 50, 100, and 150 mg per liter and the second factor includes the effect of Au-Ag nanoparticles Was on physiological parameters of Mentha piperita under salt stress. Stress was applied at the vegetative growth stage. The high amount of antioxidant compounds in menthachr('39')s extract provides a possibility of nanoparticle synthesis by mentha extract. In this study, Au-Ag alloy nanoparticles (NPs) which have consolidated properties of nanoparticles of Au and Ag synthesized by mentha extracts (green chemistry method (.The results of experiments on the Mentha piperita showed that salinity stress causes decrease length of root and shoot, fresh and dry weight of leaf and root, chlorophyll a and b, carotenoids,and anthocyanin content. Results also show that it causes increase activity of Catalase and Guaiacol peroxidase enzymes, soluble sugars amount in leaves and roots, and these effects show the greatest difference at a concentration of 150mM NaCl.