Genetic Diversity of Bread Wheat (Triticum aestivum L.) Genotypes Using RAPD and ISSR Molecular Markers

Study of Genetic Diversity in Wheat (triticum Aestivum L.) Using Random Amplified Polymorphic Dna (rapd) Markers

Twelve wheat genotypes developed through hybridization programme were screened for genetic diversity through RAPD marker. A total of 102 loci were amplified with 14 primers out of which 91 (89.2%) were polymorphic and only 11(10.8%) were monomorphic. Fragments size ranged from 142bp-5.3kb and fragments produced by various primers ranged from 1-11 with an average of 7.1 fragments per primer. The...

Assessment of Genetic Diversity of Bread Wheat (Triticum aestivum L.) Cultivars using Microsatellite Markers

Determining the level of genetic diversity and relationships among genotypes is foundation for identifying the appropriate parents in different breeding objects. In present study, the genetic diversity of 22 wheat cultivars were tested using 22 pairs of SSR primers. Out of 24 detected alleles, 11 were able to show desirable polymorphism. In average 2.18 alleles per locus were generated, in whic...

Genetic Diversity and Linkage Disequilibrium in Chinese Bread Wheat (Triticum aestivum L.) Revealed by SSR Markers

Two hundred and fifty bread wheat lines, mainly Chinese mini core accessions, were assayed for polymorphism and linkage disequilibrium (LD) based on 512 whole-genome microsatellite loci representing a mean marker density of 5.1 cM. A total of 6,724 alleles ranging from 1 to 49 per locus were identified in all collections. The mean PIC value was 0.650, ranging from 0 to 0.965. Population structu...

Comparative Analyses of the Genetic Diversity Among Bread Wheat Genotypes Based on RAPD and SSR Markers

Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers

In this study, genetic diversity of 20 wheat genotypes was evaluated using 126 simple sequence repeats (SSR) alleles, covering all three wheat genomes. A total of 1557 allelic variants were detected for 126 SSR loci. The number of alleles per locus ranged from 4 to 19 and the allelic polymorphism information content (PIC) varied from 0.66 (Xgwm429) to 0.94 (Xgwm212 and Xgw...

Evaluation of Genetic Diversity among Some Common Fig Using RAPD and ISSR Molecular Markers

The common fig (Ficus carica L.), one of the most important fruit species, belongs to Moraceae family and is widely distributed in Iran. In this study, genetic variations among some genotypes of common fig collected from six different regions of Ilam province (Iran) were evaluated based on RAPD and ISSR markers. A total of 73 and 29 alleles were produced by 14 RAPD (with their sizes ranging fro...