Expression pattern analysis of TomPRO2 and LaPA1 genes in tomato under in vitro salt stress by Semi-quantitative RT-PCR

Salt-related Genes Expression Pattern in Salt-Tolerant and Salt-Sensitive Cultivars of Cotton (Gossypium sp.) under NaCl Stress

Salinity is one of the most important limitation factors in development of agricultural products. Cotton has a relative tolerance to salinity; however, salinity reduces its growth during germination and seedling stages. In this research, split-factorial design of time based on randomized complete block design with 3 replications was used. The real-time PCR results for, root, stem, and leaves of...

in vitro semi-quantitative determinataion of human gamma-interferon expression by rt-pcr

secreted cytokines of th1 (t-helper)/th2 cells play an important role in the pathogenesis of many diseases. th1 cells secrete predominantly ifn-γ and il-2 which regulate cell-mediated immunity against intracellular pathogens and tumors. in this study, expression of ifn-γ was studied using semiquantitative rt-pcr. in brief, lymphocytes of a healthy donor were stimulated with pha (1μg/106 cell/ml...

Quantitative expression analysis of P5CS and BADH genes in cultivated wheat plants under salt and ABA treatments

Plants encounter various stresses such as drought and salinity which adversely affect growth, development and crop productivity. The expression of the genes Delta -1- pyr-roline – 5 - carboxylate synthetase (P5CS) and Betaine aldehyde dehydrogenase (BADH) extends throughout various protec-tive mechanisms in plants and allows them to adapt to unfavorable environmental condi-tions. P5CS and BADH ...

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)

Selection of suitable reference genes for quantitative RT-PCR normalization in the halophyte Halostachys caspica under salt and drought stress

The plants are always subjected to various environmental stress, because of plant sessile growth. qRT-PCR is a sensitive and reliable technology, and the normalization of target gene expression with suitable reference genes is very important for obtaining accurate data. Halostachys caspica is an extremely salt-tolerant halophyte belonging to Chenopodiaceae and a good candidate to explore the st...