Expression of miR-485-3p and its Target FOXD3 in Chronic Lymphocytic Leukemia

نویسندگان

  • Faezeh Namazi Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
  • Fatemeh Ketabchi Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
  • Mansour Moghimi Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
  • Nasrin Hadi Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran.
چکیده مقاله:

Background and Aims: Previous investigations have revealed that microRNAs (miRNAs) can function as oncogenes or tumor suppressors in chronic lymphocytic leukemia (CLL) and that the expression of miRNAs, such as miR-485-3p changes in several illnesses. This study was designed to determine the expression level of miR-485-3p and its target FOXD3 in CLL patients and controls to identify if this miRNA has the potential to be considered as a biomarker in CLL. Materials and Methods: A total of 35 CLL patients and the same number of controls were recruited. Following total RNA extraction and cDNA synthesis, quantitative analysis was performed by real-time polymerase chain reaction (PCR) using the SYBR Green PCR Master Mix to determine the expression level of miR-485-3p and its target. Moreover, in silico molecular enrichment analysis was conducted on targetome of miR-485-3p. Results: It was observed that the expression level of miR-485-3p was upregulated in CLL patients compared with controls (p<0.009). However, the expression profile of miR-485-3p’s target (FOXD3) was significantly downregulated in patients in comparison with the control group (p<0.02). Conclusions: Based on the results, miR-485-3p could be a novel biomarker to diagnose CLL and to design novel CLL control strategies by targeting FOXD3.

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عنوان ژورنال

دوره 7  شماره 1

صفحات  23- 29

تاریخ انتشار 2020-01

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