Expression of Gsk-3β And β-Catenin Proteins in the PMSG Stimulated Rat Ovary
نویسندگان
چکیده مقاله:
Purpose: The ovary is an example of a developing tissue in which developmental prosses occur throughout reproductive life. We investigate the expression of GSK-3β and β-catenin- Wnt pathway molecules- in the rat ovary during follicular development. Materials and Methods: To induce follicular growth and development, 23 days old immature female rats were injected with 10 IU of PMSG. Forty and forty-eight hours after stimulation with PMSG, the sera was collected for hormone assay, and the ovary were dissected and prepared for immunohistochemistry (IHC( and western blot(WB). Results: Our IHC and WB analysis showed that the expression of pGSK-3β and active β-catenin was increased in PMSG primed rats in compared to those of intact groups. However, the total GSK-3β and β-catenin proteins remain unchanged. Conclusion: The results indicate this idea that gonadotropin (PMSG), stabilized β-catenin in GSK-3β independent manner in the rat ovary during the follicular development.
منابع مشابه
The Effect of Mesenchymal Stem Cell and Aerobic Exercise on the Expression of β-catenin and GSK-3β Genes in Heart Tissue of Rats in the Experimental Model of Knee Osteoarthritis
Introduction: Proliferation has long been the main source of mesenchymal stem cells (MSCs) in tissue repair , cell therapy and tissue engineering strategies. On the other hand, regular exercise as part of a person’s daily routine may help manage pathological conditions. The aim of this study was to investigate the effect of mesenchymal stem cell injection and aerobic exercise on the expression ...
متن کاملAge-related neuroinflammation and changes in AKT-GSK-3β and WNT/ β-CATENIN signaling in rat hippocampus
Aging is a multifactorial process associated with an increased susceptibility to neurodegenerative disorders which can be related to chronic inflammation. Chronic inflammation, however, can be characterized by the persistent elevated glucocorticoid (GCs) levels, activation of the proinflammatory transcription factor NF-кB, as well as an increase in cytokines. Interestingly, both NF-кB and cytok...
متن کاملTGF-β1 enhanced myocardial differentiation through inhibition of the Wnt/β-catenin pathway with rat BMSCs
Objective(s): To investigate and test the hypotheses that TGF-β1 enhanced myocardial differentiation through Wnt/β-catenin pathway with rat bone marrow mesenchymal stem cells (BMSCs).Materials and Methods: Lentiviral vectors carrying the TGF-β1 gene were transduced into rat BMSCs firstly. Then several kinds of experimental methods were u...
متن کاملGSK-3β suppresses HCC cell dissociation in vitro by upregulating epithelial junction proteins and inhibiting Wnt/β-catenin signaling pathway
Glycogen synthase kinase-3β (GSK-3β) is required in the expression of epithelial junction proteins. It was found downregulated in hepatocellular carcinoma (HCC) tissues. The purpose of this study was to investigate the role of GSK-3β in modulating the metastatic behaviors of human HCC cell lines in vitro. In this study, the expression level of GSK-3β was measured in 4 human HCC cell lines, and ...
متن کاملIschemic preconditioning increases GSK-3β/β-catenin levels and ameliorates liver ischemia/reperfusion injury in rats.
Ischemic preconditioning (IPC) ameliorates ischemia/reperfusion (I/R) injury in a number of organs, and the glycogen synthase kinase-3β (GSK-3β)/β-catenin signaling pathway regulates I/R-induced proliferation and apoptosis in the central nervous system and heart. However, the function of this signaling pathway in IPC during liver I/R remains unclear. Thus, in this study, we aimed to investigte ...
متن کاملFluoxetine Regulates Neurogenesis In Vitro Through Modulation of GSK-3β/β-Catenin Signaling
BACKGROUND It is generally accepted that chronic treatment with antidepressants increases hippocampal neurogenesis, but the molecular mechanisms underlying their effects are unknown. Recently, glycogen synthase kinase-3 beta (GSK-3β)/β-catenin signaling was shown to be involved in the mechanism of how antidepressants might influence hippocampal neurogenesis. METHODS The aim of this study was ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 8 شماره 30
صفحات 0- 0
تاریخ انتشار 2010-05
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
کلمات کلیدی برای این مقاله ارائه نشده است
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023