Evaluation of the Toxicity Effects of Silk Fibroin on Isolated Fibroblast and Huvec Cells

Emerging line research showed that silk nanoparticles (NPs) have toxicity on the fibroblastand Huvec cells without any toxicity recognized mechanisms. Recently, it suggested peripheralarterial disease confounds almost eight million Americans. Also, due to the main effect offibroblast in a production of extracellular matrix (ECM), adhesive molecules, glycoproteinsand various cytokines, it decided to define the toxicity mechanistic of silk NPs in fibroblastand Huvec cells based on oxidative stress markers. Therefore, it investigated whether silkNPs is able to induce any abnormality in the fibroblast and Huvec cells based on reliable anddocumented oxidative stress methods. Our results indicated that silk NPs (0.5, 1 and 2 mg/mL)induces cellular and mitochondrial dysfunction including an increase in ROS production, lipidperoxidation, mitochondria membrane potential (MMP) collapse, and oxidation of thiol groupswhich caused to cytochrome c release. Besides, lysosomal integrity damage and decreased inATP/ADP ratio proposed disruptive effect of silk NPs on the mitochondrial respiratory chainand cell death signaling induction.