Evaluation of six cold-season turfgrasses responses to lead phytotoxicity for screening tolerant species

Lead (Pb) is one of the most hazardous heavy metal that caused serious problems for ecosystem at recent years. In this study, the effect of Pb on seed germination, incipient growth and antioxidant enzymes of six common used turfgrasses was investigated. The results showed that after implementation of different concentrations (0, 1000, 2000 and 3000 µM) of Pb, Agropyron elongatum exhibited the highest germination percentage (90%) under 3000 µM Pb, followed by Lollium perenne L. (85.56%). The lowest germination parameters including germination vigor, rate, index and germination percentage was found in Festuca ovina, which means it is the most sensitive species to Pb than the other studied turfgrasses. In contrast, F. rubra, F. ovina, A. elongatum and L. perenne had the most significant aerial growth and the lowest chlorophyll degradation even at high lead concentration. An increase in L. perenne and A. elongatum antioxidant enzymes activities as well as a decrease in F. ovina observed after exposure to 3000 µM Pb which correlated to their tolerance and sensitivity to Pb respectively. However, such increase in antioxidant activity was also found in F. Rubra and Poa pratensis but could not inhibit chlorophyll degradation and seedling growth retardation. It might support that there is suggesting the probable existence of a lead-injury mechanism rather than the oxidative stress. Overall, A. elongatum is introduced as the most tolerant turfgrass among studied ones, which could be cultivated in Pb-polluted sites.