Evaluation of Cefoxitin Disk Diffusion Test for Routine Detection of Methicillin-resistant Staphylococcus Aureus
نویسندگان
چکیده مقاله:
Background and Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial and community acquired infections. Detection of MRSA in laboratories is very important for treatment and appropriate infection control. The aim of this study was to evaluate cefoxitin disk diffusion method for detection of MRSA and comparison of this method with other conventional methods. Methods: A total of 175 clinical isolates of S. aureus isolated from clinical specimens were studied. The isolates were identified by conventional laboratory methods. In this respect, E-test MIC, cefoxitin and oxacillin disk diffusion methods, and MAST ID Methicillin strips were used for detection of MRSA. All disk diffusion methods were performed as recommended by NCCL and manufacturers’ guidelines. Results: Using E-test MIC, 53 out of 175 strains of S. aureus were resistant to methicillin. In addition, disk diffusion method using oxacillin disk showed that 52 strains are resistant to methicillin. In this respect, 8 strains had intermediate resistance to methicillin. For cefoxitin disk diffusion method, 52 strains were resistant to methicillin. This method had a good correlation with E-test MIC method. Meanwhile, MAST ID methicillin strips detected 47 strains that were resistant to mehicillin. Sensitivity and specificity for both cefoxitin and oxacillin disk diffusion methods were 98%and 100% respectively. However cefoxitin was better than oxacillin for detecting intermediate resistant strains of S. aureus. Sensitivity and specificity for MAST ID methicillin strips were 91% and 100% respectively. Conclusion: This study revealed that cefoxitin disk diffusion method is a good alternative for oxacillin disk diffusion method for detection of MRSA. This method is more reliable for identification of intermediate resistant strains of S. aureus.
منابع مشابه
evaluation of cefoxitin disk diffusion test for routine detection of methicillin-resistant staphylococcus aureus
background and objectives: methicillin-resistant staphylococcus aureus (mrsa) is a major cause of nosocomial and community acquired infections. detection of mrsa in laboratories is very important for treatment and appropriate infection control. the aim of this study was to evaluate cefoxitin disk diffusion method for detection of mrsa and comparison of this method with other conventional method...
متن کاملEvaluation of oxacillin and cefoxitin disk diffusion test for routine detection of methicillin resistant Staphylococcus aureus.
متن کامل
Evaluation of a cefoxitin disk diffusion test for the routine detection of methicillin-resistant Staphylococcus aureus.
Two oxacillin disk methods were compared with a cefoxitin disk diffusion test for detection of methicillin-resistant Staphylococcus aureus (MRSA), with PCR for mecA as the reference method. When tested with 115 MRSA and 350 methicillin-susceptible S. aureus isolates, the cefoxitin disk test (specificity 100%, sensitivity 96.5%) was superior to the oxacillin disk methods (specificity 99.1%, sens...
متن کاملDetection of Methicillin Resistant Staphylococcus aureus (MRSA) by CHROMagar Versus Cefoxitin Disk Diffusion Method
Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene to detection of MRSA. A total of 220 clinical isolates of S. aureus which w...
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عنوان ژورنال
دوره 1 شماره 4
صفحات 145- 148
تاریخ انتشار 2006-09-01
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