Enhanced Bioadsorption of Cadmium and Nickel by E. coli Displaying A Metal Binding Motif Using CS3 Fimbriae

Development of bacterium-based heavy metal biosorbents: enhanced uptake of cadmium and mercury by Escherichia coli expressing a metal binding motif.

A gene coding for a de novo peptide sequence containing a metal binding motif was chemically synthesized and expressed in Escherichia coli as a fusion with the maltose binding protein. Bacterial cells expressing the metal binding peptide fusion demonstrated enhanced binding of Cd2+ and Hg2+ compared to bacterial cells lacking the metal binding peptide. The potential use of genetically engineere...

Bacterium-based heavy metal biosorbents: enhanced uptake of cadmium by E. coli expressing a metallothionein fused to beta-galactosidase.

We investigated the potential utility of a recombinant E. coli that expresses the human metallothionein II gene as a fusion protein with beta-galactosidase as a heavy metal biosorbent. E. coli cells expressing the metallothionein fusion demonstrated enhanced binding of Cd2+ compared to cells that lack the metallothionein. It was shown that the metallothionein fusion was capable of efficiently r...

Phytoremediation of cadmium and nickel using Vetiveria zizanioides

an investigation into translation of cultural concepts by beginner and advance student using think – aloud protocols

this research aims at answering the questions about translation problems and strategies applied by translators when translating cultural concepts. in order to address this issue, qualitative and quantitative study were conducted on two groups of subjects at imam reza international university of mashhad. these two groups were assigned as beginner and advanced translation students (10 students). ...

development of different optical methods for determination of glucose using cadmium telluride quantum dots and silver nanoparticles

a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...

Broad Wavelength Range Metal-Enhanced Fluorescence Using Nickel Nanodeposits

We describe the use of surface-deposited nickel nanoparticles to enhance the fluorescence signatures of fluorophores. Different density Ni nanoparticulate substrates were fabricated and characterized using both AFM and optical absorption techniques. When fluorophores were placed in close proximity to the substrates, metal-enhanced fluorescence (MEF) was observed. The wavelength dependence of th...