EFFECTS OF UBIQUINOL-1 0 AND f3-CAROTENE ON THE IN VITRO SUSCEPTIBILITY OF LOW-DENSITY LIPOPROTEIN TO COPPER-INDUCED OXIDATION

 ABSTRACT Background: Dietary antioxidant intake has been reported to be inversely associated with coronary miery disease. To clarify the possible role oflipophilic antioxidants in the prevention of atherosclerosis, we investigated the effects ofubiquinol-1 0 and ~-carotene on the susceptibility oflow-density lipoprotein(LD L) to oxidative modification. Methods: In this study, first "ubiquinol-1 0 and ~-carotene" were added to plasma and incubated for 3hr at 37°C. Then, the LDL fraction was separated by ultracentrifugation. The oxidizability ofLDL was estimated by measuring conjugated diene (CD), lipid peroxides and thiobarbituric acid-reactive substances (TBARS) after cupric sulfate solution was added. Results: We showed that ubiquinone-! 0 and ~-carotene significantly (p ~-carotene. Conclusion: LDL exposed to the lipophilic antioxidants in vitro reduced oxidizability. These findings suggest that ubiquinol-1 0 and ~-carotene have a role in ameliorating atherosclerosis.