Effects of Different Doses of Progesterone on Motion Parameters of Mouse Spermatozoa During the Course of Hyperactivation

Purpose: To evaluate the changes that occur in the motility parameters of progesterone treated sperms during the course of hyperactivation. Materials and Methods: Spermatozoa treated with different doses of progesterone were videotaped after 10 min and 90 min. For each sperm, 1 sec. of movement of the head-midpiece junction was traced from the videotape. For each tracing, seven motility parameters were studied using computer assisted image analysis. Results: For all the progesterone treated spermatozoa, motility rates differed significantly from that of the control group after 90 occurred during 10 min. of incubation. With treatment of 1mg/ml progesterone, the hyperactivated motility pattern of spermatozoa occurred 90 min. after incubation similar to that in the control group, showing that a low dose of progesterone is unable to induce hyperactivation. Conclusion: Progesterone induces hyperactivation in mouse sperm and reduces the motility rate during the time.