Effects of bilateral tubal sterilization on serum oxidative stress and ovarian hormones in female rats

Introduction: Tubal Sterilization (TS) is one of the most common contraceptive methods. Some researchers believe that TL has complications, whereas there are contradictory reports about TS effects on ovarian function. The aim of this study was to evaluate the indices of oxidative stress and survey ovarian function after TS. Methods: The study groups consisted of 63 Sprague Dawley female rats. The study comprised of 2 protocols A and B. A Protocol: survey oxidative stress and ovarian hormones and B protocol: study of estrous cycle. Each of the protocols included control, sham and Tubal Sterilization (Pomeroy) groups. In protocol A, rats of PAB (Prooxidant-Antioxidant Balance), MDA (Malondialdhyde) 17 beta estradiol and progestrone were measured 15 and 45 days after intervention. In protocol B, 15 and 45 days after intervention, animal&rsquo;s estrous cycle was followed for 15 days. Data were analyzed by means of SPSS. Software, using ANOVA and P<0.05 was considered statistically significant. Results: Our results showed that PAB and MDA significantly increased and estradiol and progesterone significantly decreased in rats undergone TS (P<0.05). Also TS caused irregularity in estrous cycle after 15 and 45 days. Conclusion: TS Increased oxidative stress level and decreased estradiol and progesterone of serum. Complications of TS may be due to increased oxidants and decreased estrogen and progesterone. By prescribing antioxidant, effects of TS can be reduced.