Effect of olfactory ensheathing cells (OECs) transplantation on functional recovery in acute phase of spinal contused rats
نویسندگان
چکیده مقاله:
Introduction: Spinal cord injuries (SCI) lead to permanent irreversible functional deficits. Poor prognosis of patients is the motivation of searching a treatment for the chronic injury. Planting stem cells provides us with a promising strategy. In the meanwhile, the use of olfactory ensheathing cells (OECs) has shown very good results. This study aims at evaluating the effect of transplanted OECs on functional recovery of acute SCI in rats. Materials and methods: In this study, eighteen adult male Wistar rats weighting approximately 210 ± 10 gr were used to study spinal cord injury. They were randomly divided into four groups: Sham (n = 3), Control (n = 6), Vehicle (n=3) and Treatment (n=6). In the Sham Group, only laminectomy was performed in the area of T9 spinal cord. In three groups of Control, vehicle and Treatment, after laminectomy, spinal cord contusion model was performed using the Weight drop technique. Immediately after the injury, 10μl Dulbecco's Modified Eagle Medium (DMEM) alone or with 106 cells were injected to vehicle and Treatment Group animals. For culturing cells, the olfactory mucosa of 7-day-old male Wistar rats was used. Motor function of animals in all groups, was evaluated in the first 48 hours daily and then weekly for eight weeks. Results: Comparing the results of the second to eighth week of the study showed significant differences in the group receiving the OECs with the control group (P<0.05). Conclusion: The results indicate a positive influence of the olfactory ensheathing cells in functional improvement of spinal cord injury in the acute phase of injury.
منابع مشابه
effect of olfactory ensheathing cells (oecs) transplantation on functional recovery in acute phase of spinal contused rats
introduction: spinal cord injuries (sci) lead to permanent irreversible functional deficits. poor prognosis of patients is the motivation of searching a treatment for the chronic injury. planting stem cells provides us with a promising strategy. in the meanwhile, the use of olfactory ensheathing cells (oecs) has shown very good results. this study aims at evaluating the effect of transplante...
متن کاملTransplantation of Olfactory Mucosa Improve Functional Recovery and Axonal Regeneration Following Sciatic Nerve Repair in Rats
Background: Olfactory ensheathing glia (OEG) has been shown to have a neuroprotective effect after being transplanted in rats with spinal cord injury. This study was conducted to determine the possible beneficial results of olfactory mucosa transplantation (OMT) which is a source of OEG on functional recovery and axonal regeneration after transection of the sciatic nerve. Methods: In this study...
متن کاملthe effect of aqueous extract of garlic on formalin-induced pain in male rats
چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...
15 صفحه اولMeta analysis of olfactory ensheathing cell transplantation promoting functional recovery of motor nerves in rats with complete spinal cord transection
OBJECTIVE To evaluate the effects of olfactory ensheathing cell transplantation on functional recovery of rats with complete spinal cord transection. DATA SOURCES A computer-based online search of Medline (1989-2013), Embase (1989-2013), Cochrane library (1989-2013), Chinese Biomedical Literature Database (1989-2013), China National Knowledge Infrastructure (1989-2013), VIP (1989-2013), Wanfa...
متن کاملRepair of Spinal Cord Injury by Co-Transplantation of embryonic Stem Cell-Derived Motor Neuron and Olfactory Ensheathing Cell
Background: The failure of regeneration after spinal cord injury (SCI) has been attributed to axonal demyelination and neuronal death. Cellular replacement and white matter regeneration are both necessary for SCI repair. In this study, we evaluated the co-transplantation of olfactory ensheathing cells (OEC) and embryonic stem (ES) cell-derived motor neurons (ESMN) on contused SCI. Methods: OEC...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 4 شماره 1
صفحات 30- 36
تاریخ انتشار 2017-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023