Effect of 810nm Diode Laser on the Number of Periopathogen in Gingival Crevicular Fluid (GCF) of Fixed orthodontics Patients without Gingival Inflammation

Abstract Background and aim: One of the most common complications in the treatment of fixed orthodontics during treatment is periodontal problems. Different therapies such as mouthwashes, antibiotics and today laser therapy are used to treat periodontal problems in orthodontic patients. The aim of  this study, is that we evaluated the effect of the Diode laser on the number of four periopathogen microorganisms. Materials and methods: In this experimental study, 12 patients with fixed orthodontic appliance without gingivitis and periodontitis were selected. The left and right second premolar teeth were considered as case and control groups. After periodontal examinations, before and after the laser, were taken GCF sample and After 2 weeks and 6 weeks after laser irradiation, samples were re-sampled and transferred to the laboratory. Data were analyzed by Wilcoxon and Mann-Whitney statistical tests Results: The mean of the four kind of preopatogen immediately after laser radiation decreased significantly. (P<0.001)And this decrease continued after two weeks, but after six weeks only the mean of the number of Prevotella intermedia  (P=0.008) and porphyrpmonas gingivali (P=0.002) decreased significantly. Conclusion: lasertherapy is effective in the reduction of the number of preopatogenic microorganisms in gingival crevicular fluid of fixed orthodontics patients without inflammation which this effect last for a shorter period in cases of Actinomyces comitans,Actinomyces odontolyticus