Determination of Sulfamethoxazole and Trimethoprim in Pharmaceuticals by Visible and UV Spectrophotometry

نویسندگان

  • F Shamsa
  • L Amani
چکیده مقاله:

A method for the simultaneous determination of sulfamethoxazole (SMX) and trimethoprim (TMP), based on a direct determination of SMX after diazotization and coupling with 2-naphthol by visible spectrophotometry and an indirect determination of TMP in the UV region by difference was developed. By the suggested procedure, it was possible to analyze SMX and TMP in pharmaceutical preparations without separating from each other or from the excipients. Primarily a portion of the sample was diluted to have an absorbance of 0.4-0.7 at 271 nm, which corresponds to both SMX and TMP. By adding sodium nitrite, HCl and 2-napthol to 1 ml of the diluted solution, only SMX is diazotized and coupled with 2-naphthol forming a colored product with ? max at 482 nm where the quantity of SMX could be obtained directly from a calibration curve. By plotting another calibration curve for SMX at 271 nm, the influence of SMX on the total absorbance of a mixture of SMX and TMP at 271 nm could be deduced. The absorbance corresponding to TMP only was obtained by difference, which was transferred to quantity using a calibration curve at 271 nm. This procedure was applied successfully for the analysis of SMX and TMP in pharmaceutical preparations without prior separation and with acceptable errors.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

determination of sulfamethoxazole and trimethoprim in pharmaceuticals by visible and uv spectrophotometry

a method for the simultaneous determination of sulfamethoxazole (smx) and trimethoprim (tmp), based on a direct determination of smx after diazotization and coupling with 2-naphthol by visible spectrophotometry and an indirect determination of tmp in the uv region by difference was developed. by the suggested procedure, it was possible to analyze smx and tmp in pharmaceutical preparations witho...

متن کامل

UV-Visible spectrophotometry of Repaglinide in bulk and in formulation by using methyl orange as reagents

Two sensitive spectrophotometric methods are presented for the assay of Repaglinide in bulkdrug and in formulations using methyl orange, as reagents. Oxidant by reacting with a fixedamount of either methyl orange and measuring the absorbance at 485.2 nm (method-I) andmeasuring the absorbance at 618.9 nm (method-II). Developed method is based on the formationof extractable colored complex of dru...

متن کامل

Determination of Triiodide Ion Concentration Using UV-Visible Spectrophotometry†

The concentration of iodine species can be measured using several different analysis methods. One of the most wellknown methods involves iodine titration with a thiosulfate solution. Total iodine can be analyzed using spectrometry, such as inductively coupled plasma-atomic emission spectrometry (ICP-AES), atomic absorption spectrometry (AAS) and inductively coupled plasma-mass spectrometry (ICP...

متن کامل

Simultaneous determination of iron, cobalt, nickel and copper by UV-visible spectrophotometry with multivariate calibration.

A method for the simultaneous spectrophotometric determination of the divalent ions of iron, cobalt, nickel and copper based on the formation of their complexes with 1,5-bis(di-2-pyridylmethylene) thiocarbonohydrazide (DPTH) is proposed. The resolution of quaternary mixtures of these metallic ions was accomplished by several chemometric approaches. A comparative study of the results obtained fo...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره Volume 5  شماره Number 1

صفحات  31- 36

تاریخ انتشار 2010-11-20

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023