Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR

نویسندگان

  • Ramazan Rajabnia Infectious Diseases & Tropical Medicine Research Center, Department of Microbiology and Immunology, Babol University of Medical Sciences, Babol, Iran.
  • Sharareh Moghim Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran.
  • Zohreh Hojati Genetics Division, Biology Department, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
چکیده مقاله:

Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain.

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determination of ctxab expression in vibrio cholerae classical and el tor strains using real-time pcr

cholera is an infection of the small intestines caused by the bacterium v. cholerae. it is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. the major virulence factor produced by v. cholerae during infection is the cholera toxin. total mrna extraction and reverse transcription was performed for making ctxab cdna. relative real-time...

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عنوان ژورنال

دوره 2  شماره None

صفحات  9- 13

تاریخ انتشار 2013-03

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