Detection of Viable But Non-Culturable State of Escherichia coli O157:H7 Using Reverse Transcription PCR

The viable but non-culturable state in pathogenic Escherichia coli: A general review

BACKGROUND The persistence and pathogenicity of pathogenic bacteria are dependent on the ability of the species to survive in adverse conditions. During the infectious process, the organism may need to pass through certain hostile anatomical sites, such as the stomach. Under various environmental stresses, many bacteria enter into the viable but non-culturable (VBNC) state, where they are 'aliv...

Viable but Non-culturable Bacteria: Clinical Practice and Future Perspective

In this editorial, the importance of VBNC in clinical practice and future researches interests has been pinpointed. Accurate detection of VBNC, especially for major mentioned pathogens, is the future research gap. 

Rapid quantification of culturable and viable-but-nonculturable Escherichia coli O157:H7 in beef products using EMA-Real Time PCR

Detection of Viable But Non - culturable Escherichia coli O 157 : H 7 in 1 Drinking Water and River Water 2

Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR.

Detection of pathogens in contaminated food products by PCR can result in false-positive data due to the amplification of DNA from nonviable cells. A new method based on reverse transcription-PCR (RT-PCR) amplification of mRNA for the specific detection of viable Listeria monocytogenes was developed. The expression of three L. monocytogenes genes, iap, hly, and prfA, was examined to determine a...

Application of Reverse Transcriptase –PCR (RT-PCR) for rapid detection of viable Escherichia coli in drinking water samples

BACKGROUND Polymerase chain reaction (PCR) is preferred to other methods for detecting Escherichia coli (E. coli) in water in terms of speed, accuracy and efficiency. False positive result is considered as the major disadvantages of PCR. For this reason, reverse transcriptase-polymerase chain reaction (RT-PCR) can be used to solve this problem. The aim of present study was to determine the effi...