Detection of Torque teno midi virus/Small anellovirus (TTMDV/SAV) in the sera of domestic village chickens and its vertical transmission from hen to eggs

نویسندگان

  • M. Bouzari Division of Microbiology, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
  • Sh. Salmanizadeh MSc Student, Division of Microbiology, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
چکیده مقاله:

Although the infection of different animals and non-human primates with other members of Anelloviridae have already been reported there is no report about infection of animals with Torque teno midi virus/Small anellovirs (TTMDV/SAV). The aim of this study was to detect the virus in domestic village chickens. Blood samples were collected from 79 domestic village chickens in Isfahan. Blood samples of five adult laying hens and one cockerel were collected in three consecutive weeks (days 1, 8 and 14) as experimental chickens. Ten eggs were randomly collected from the eggs laid during days 12 to 17 and thin and thick egg whites and yolk samples were collected aseptically. After DNA extraction Nested-PCR was performed using SMAs/SMAr primers. In PCR, 431 bp and 441 bp products were detected. The detected bands were extracted and sequenced. Totally 26 out of 79 (32.9%) of the blood samples were positive for the virus. The frequency of the infection of the different parts of the eggs tested was 76%. For the first time TTMDV/SAV was detected in domestic village chickens which also vertically transmitted to eggs.

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منابع مشابه

Detection of Torque teno midi virus/Small anellovirus (TTMDV/SAV) in the sera of domestic village chickens and its vertical transmission from hen to eggs.

Although the infection of different animals and non-human primates with other members of Anelloviridae have already been reported there is no report about infection of animals with Torque teno midi virus/Small anellovirs (TTMDV/SAV). The aim of this study was to detect the virus in domestic village chickens. Blood samples were collected from 79 domestic village chickens in Isfahan. Blood sample...

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detection of torque teno midi virus/small anellovirus (ttmdv/sav) in the sera of domestic village chickens and its vertical transmission from hen to eggs

although the infection of different animals and non-human primates with other members of anelloviridae have already been reported there is no report about infection of animals with torque teno midi virus/small anellovirs (ttmdv/sav). the aim of this study was to detect the virus in domestic village chickens. blood samples were collected from 79 domestic village chickens in isfahan. blood sample...

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15 صفحه اول

Detection of torque teno virus (TTV) in domestic village chickens in Iran

Torque teno virus (TTV) is prevalent worldwide and has been extensively studied in human and some wild and domestic animals. As the studies on TTV in chickens was rare and there was no information about the infection of domestic village chickens with TTV and also structural resemblance of this virus to chicken anemia virus, the frequency of the infection in domestic village chickens in differen...

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Detection of torque teno virus (TTV) in domestic village chickens in Iran

Torque teno virus (TTV) is prevalent worldwide and has been extensively studied in human and some wild and domestic animals. As the studies on TTV in chickens was rare and there was no information about the infection of domestic village chickens with TTV and also structural resemblance of this virus to chicken anemia virus, the frequency of the infection in domestic village chickens in differen...

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detection of torque teno virus (ttv) in domestic village chickens in iran

torque teno virus (ttv) is prevalent worldwide and has been extensively studied in human and some wild and domestic animals. as the studies on ttv in chickens was rare and there was no information about the infection of domestic village chickens with ttv and also structural resemblance of this virus to chicken anemia virus, the frequency of the infection in domestic village chickens in differen...

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عنوان ژورنال

دوره 16  شماره 1

صفحات  110- 113

تاریخ انتشار 2015-03-30

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