Comparative evaluation of apical debris extrusion between RaCe and Komet F6 An Ex-Vivo Study

Background & Aim: Apical extrusion of debris is one of the reasons for flare-up(pain,swelling,or both). This study aimed to determine the apical debris extrusion of two rotary systems(RaCe and Komet F6 SkyTaper) in curved mesiobuccal canals of mandibular first molars. Material and methods: Thirty mandibular first molars (with mesiobuccal canal curvature 25-35 degree) were included in this experimental study and divided randomly  into two experimental groups.Root canals in group 1 prepared with RaCe and group 2 prepared with Komet F6 SkyTaper system.The roots were fixed in preweighted vials in order that extruded debries accumulate in lower chamber, after desiccation of the chamber exact weight of debris were evaluated. The datas were analysed by T-test (p<0.05). Results: The mass debris extruded weight in RaCe group was 1.76±0.925 g, while in Komet group was 1.73±0.02909 g and the differences was insignificant ( p= 0.2).  Conclusions:It seems, Race and Komet F6 systems have not singinficant differences considering the extruded debreis in preparation of dental canal procedures. Background & Aim: Helicobacter pylori is a major cause of gastric ulcer and peptic ulcer and is considered a risk factor for gastric cancer. Gingival grooves in the teeth of people with chronic periodontitis may act as a reservoir for H. pylori. Therefore, the aim of this study was to investigate the virulence genes of H. pylori isolated from dental plaques. Material & Methods: The study was cross-sectional and from 120 patients with periodontitis referring to Tehran dental clinics, 111 dental plaque samples were isolated. Sterile physiology serum was used to transfer the sample. Growth in Brucella agar + 1% starch + 10-7% at 37 ° C and 95% humidity and CO2 concentration of 0.5% in CO2 incubator. In order to identify the molecules, DNA extraction was performed and then the sequences of JW22 and JW23 regions were used for accurate identification. Results: Of the 111 dental plaque samples taken from 120 participants in this study, 22 strains (19%) were positive for H. pylori which 10 strains carrying different H. pylori virulence genes. The molecular analysis of the studied genes showed that the frequency of cagA, cagT, cagE, vacA and hrgA genes showed that the prevalence of these genes was 19% of H. pylori strains of 3 (57.5%), 1 (0.19%), %), 2 (0.34%), 1 (19.1%) and 1 (0.19%), respectively. Conclusions: It seems that in spite of the presence of Helicobacter pylori virulence genes in the dental plaque, to control the related diseases in addition to antibiotic treatments periodontal treatments and dental plaque control are also required.   Key words:  Extrusion of debries , Komet F6 SkyTaper , RaCe