Biofilm Formation, Antimicrobial Resistance and Biofilm-Related Genes among Uropathogens Isolated from Catheterized Uro-Oncology Patients
نویسندگان
چکیده مقاله:
Background and Objective: Despite the critical importance of catheter as an indwelling medical device, its prolonged utilization in hospitalized patients may lead to infection. This study aimed to identify distribution of uropathogenic bacteria isolated from catheterized uro-oncology patients, their biofilm production, and antimicrobial resistance patterns to generally -used antibiotics. Materials and Methods: The urine samples of catheterized urology cancer patients were collected for urinalysis and urine culture. Then capability of biofilm production was detected by Congo red agar method, tube method, and microtiter plate assay. Antimicrobial susceptibility test was also performed using the Kirby–Bauer disc diffusion method on Muller–Hinton agar. Subsequently, polymerase chain reaction (PCR) assays were used to detect the biofilm encoding genes. Results: Of the 100 urinary catheter samples, 76 isolates were recovered from urinary catheters of 52 patients. Escherichia coli was established to be the most frequent pathogen isolated from the urine of patients followed by Pseudomonas and Staphylococcus. All of Pseudomonas aeruginosa and Methicillin-resistant Staphylococcus aureus (MRSA) isolates were found to be biofilm producers. All studied isolates were found resistant to ampicillin, amoxicillin, and cephalexin. All biofilm- producer MRSA and Pseudomonas isolates were found to harbor the virulence genes studied. Both imipenem and fosfomycin were the most effective antibiotics against isolated bacteria. Conclusion: In our study virulent pathogens with highly- resistant profile and potential to form biofilm were isolated from uro-oncology patients. Therefore, the current study highlights the significance of antibiotic resistance which can lead to treatment failure.
منابع مشابه
Biofilm formation and antimicrobial resistance in methicillin-resistant Staphylococcus aureus isolated from burn patients, Iran.
INTRODUCTION Burns are the most serious forms of trauma and a major cause of mortality worldwide. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common pathogens of burn wound infections; treatment has faced serious problems due to antibiotic resistance in these strains. Biofilm formation, which increases antibiotic resistance capabilities and is considered to be a virule...
متن کاملBiofilm Production among Uropathogens and Their Antibiogram
Urinary tract infection (UTI) is the most commonly acquired bacterial infection. Antibiotic resistance of uropathogens has been known to increase worldwide and biofilm production being the prime cause. The objective of this study was to detect the production of biofilm by uropathogens isolated from UTIs and their antimicrobial susceptibility pattern. A total 570 urine samples from clinically su...
متن کاملAntiobiotic resistance pattern of biofilm-forming uropathogens isolated from catheterised patients in Pondicherry, India.
BACKGROUND Microbial biofilms pose a public health problem for persons requiring indwelling medical devices, as micro-organisms in biofilms are difficult to treat with antimicrobial agents. Thus the present study includes biofilm formation and antibiotic resistance pattern of uropathogens in hospitalised patients with catheter associated urinary tract infections (UTI). METHOD This prospective...
متن کاملHigh Frequency of biofilm related genes (icaD, fnbA, icaA and clfA) among Staphylococcus aureus isolated from bovine with subclinical mastitis
Background and aim: Regarding to the importance of Staphylococcus aureuscolonization in bovine mastitis and the role of biofilm in its pathogenesis, the aim of the present study were to assessment of biofilm production and identification of biofilm related genes among S. aureus isolated from bovine with subclinical mastitis. Materials and Methods: In this cross-sectional study, a total of 502 m...
متن کاملModeling biofilm antimicrobial resistance.
A computer model capable of integrating mechanisms of biofilm resistance to disinfection by antimicrobial agents was developed. Resistance mechanisms considered included retarded penetration due to a stoichiometric reaction between the antimicrobial agent and biomass, incomplete penetration due to a catalytic reaction between the antimicrobial agent and the biomass, and the existence of a fract...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 30 شماره None
صفحات 7- 7
تاریخ انتشار 2022-07
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023