APPLICATION OF DOT IMMUNOASSAY (DIA) FOR DETECTION AND TITRATION OF MEASLES VIRUS ANTIBODIES

application of dot immunoassay (dia) for detection and titration of measles virus antibodies

measles is one of the most contagious human diseases. although mass vaccination programs have reduced the incidence of this disease, measles is still an important cause of morbidity and mortality among children in developing countries. therefore the use of sensitive techniques to evaluate vaccine efficacy and level of immunity among members of susceptible communities is crucial. serum neutraliz...

APPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY

A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate ...

Detection and titration of measles virus antibody by hemagglutination inhibition and by dot immunobinding.

Measles continues to be a major disease of both human and nonhuman primates. The dot immunobinding assay, a modified enzyme immunoassay, permits the detection of measles virus antibody in the nonlaboratory setting with either serum or whole blood collected on filter paper.

Standardization of ELISA for Detection of Avian Influenza Virus Antibodies

Dot-Blot Enzyme Immunoassay for the Detection of Bovine Herpes Virus-1(BHV-1) Antibodies

Various detection methods have been developed for serological monitoring BHV1, an alphaherpesvirus and the cause of the respiratory disease, abortion, conjectivitis and other clinical forms of disease complex in cattle, in different laboratories throughout the world. The objective of this study was to develop and evaluate dot-blot enzyme immunoassay as a proper and inexpensive method for detect...

application and construction of carbon paste modified electrodes developed for determination of metal ions in some real samples

ساخت الکترودهاِی اصلاح شده ِیکِی از چالشهاِی همِیشگِی در دانش شیمِی بوِیژه شیمِی تجزیه مِی باشد ،که با در نظر گرفتن سادگِی ساخت، کاربردی بودن و ارزان بودن روش مِی توان به باارزش بودن چنِین سنسورهاِی پِی برد.آنچه که در ادامه آورده شده به ساخت و کاربرد الکترودهاِی اصلاح شده با استفاده از نانو ذرات در اندازه گِیرِی ولتامترِی آهن وکادمِیم اشاره دارد. کار اول اختصاص دارد به ساخت الکترود خمِیر کربن اصلاح شده با لِیگاند داِ...