Anticonvulsant studies on a traditional antiepileptic mixture used by the Hausa people of north-western Nigeria

Background and objectives: The use of herbal drugs in the treatment of many neurological disorders is gaining popularity in developing countries due to their fewer unwanted side effects, affordability and cultural acceptability. A mixture of three plants roots Calotropis procera (Asclepediaceae), Combretum micranthum (Combretaceae) and Ficus abutilifolia (Moraceae) has been reported in Hausa traditional treatment of epilepsy. We have reported the evaluation of the acute toxicity and anticonvulsant activity of the ethanol extract of this mixture. Methods: The intra-peritoneal medial lethal dose (LD50) of the aqueous ethanol extract of the mixture as well as its anticonvulsive activity against pentylenetetrazole (PTZ), 4-amino pyridine (4-AMP) and maximum electric shock (MES) were evaluated. Results: The mixture at the doses of 25 to 100 mg/kg could not afford a significant protection to mice against PTZ and 4-AMP; however, it significantly delayed the mean onset and reduced the mean recovery time of the animals at the tested doses in 4-AMP test (p<0.05). Furthermore, it afforded a dose-depended protection to one-day-old cockerels against MES. The LD50 of the extract in albino mice was estimated to be greater than 1000 mg/kg. Conclusion: The present study suggest some level of protection by the extract of the traditional mixture against MES-induced seizure in chicks, thereby giving support to the traditional claim for its application in the treatment and/or management of convulsion and epilepsy.