Analysis of Herpes Simplex Virus in Suspected Encephalitis, Keratitis and Dermal Infections Using Real- Time PCR

diagnosing of herpes simplex virus infections in suspected patients using real-time pcr

background herpes simplex virus infections are very common worldwide. the virus can cause infection in various body parts, especially eyes and the nervous system. therefore, an early diagnosis and highly sensitive method is very helpful. objectives the present study sought to investigate the efficiency of real-time taqman probe pcr in the diagnosis of hsv infection in suspected patients. patien...

Diagnosing of Herpes Simplex Virus Infections in Suspected Patients Using Real-Time PCR

BACKGROUND Herpes simplex virus infections are very common worldwide. The virus can cause infection in various body parts, especially eyes and the nervous system. Therefore, an early diagnosis and highly sensitive method is very helpful. OBJECTIVES The present study sought to investigate the efficiency of Real-time TaqMan probe PCR in the diagnosis of HSV infection in suspected patients. PA...

Tzanck smears in herpes simplex virus infections

Background: The diagnosis of herpes simplex virus may requirevirological confirmation. Tzanck smear is an old, rapid, costeffective but nonspecific method that has been recently re-evaluatedas a method for the diagnosis of herpes virus infection. This studywas conducted to compare Tzanck smear and viral culture in thediagnosis of herpes simplex virus infection in patients clinicallysuspected to...

Real-Time PCR and Varicella-Zoster Virus Infections by Rapid Detection of Herpes Simplex Virus

Rapid detection of herpes simplex virus and varicella-zoster virus infections by real-time PCR.

The herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2) and varicella-zoster virus (VZV) can cause life-threatening infections of the central nervous system and lead to severe infections in immunocompromised subjects and newborns. In these cases, rapid diagnosis is crucial. We developed three different real-time PCR assays based on TaqMan chemistry for the LightCycler instrument to detect HS...

Detection of Herpes simplex virus DNA by real-time PCR.

Molecular detection of herpes simplex virus (HSV) DNA is recognized as the reference standard assay method for the sensitive and specific diagnosis of central nervous system infections caused by HSV. In this study, a molecular assay based on real-time PCR on the LightCycler (LC) instrument was evaluated and compared with a home-brew molecular assay. The detection limit of the LC assay was deter...