An Improved GC Method for Rapid Analysis of Valproic Acid in Human Plasma Without Derivatization

نویسندگان

  • Abbas Kebriaeezadeh Department of Toxicology and Pharmacology, Pivot of Excellency Center for Toxicology and Food Chemistry, Faculty of Pharmacy, and Pharmaceutical Science Research Center, University of Tehran/ Medical Sciences, Tehran, Iran
  • Farzad Kobarfard Department of Medicinal Chemistry, Faculty of Pharmacy
  • Hossein Pakdaman Department of Neurology, Loghman Hospital, Faculty of Medicine, Shaheed Beheshti University of Medical Sciences, Tehran, Iran
  • Noushin Rastkari Center for Environmental Research,Tehran University of Medical Sciences, Tehran, Iran
  • Omid Ahmadkhaniha Doping Control Laboratory, Sports Medicine Federation of Iran, Tehran, Iran
  • Reza Ahmadkhaniha Department of Medicinal Chemistry, Faculty of Pharmacy
چکیده مقاله:

        A simple, reproducible and rapid gas chromatographic method for precise determination of valproic acid (VPA) in human plasma has been developed. Total time for sample preparation and GC analysis is less than 45 min. After plasma protein precipitation, VPA was extracted into chloroform with suitable recovery. By using Stabilwax®-DA capillary GC column, a symmetrical gas chromatographic peak was obtained without the need for derivatization. The calibration curve was proved to be linear (r2 = 0.998) in a wide concentration range (0.45-100 μg/ml). Inter-day and intra-day accuracy and precision of this method was investigated during the method validation and the method has good precision and accuracy. This method is highly reproducible with a limit of detection 150 ng/ml of VPA in human plasma and could be used in TDM and pharmacokinetic studies.

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an improved gc method for rapid analysis of valproic acid in human plasma without derivatization

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عنوان ژورنال

دوره 3  شماره 1

صفحات  37- 42

تاریخ انتشار 2007-01-01

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