A Reverse transcription-PCR assay for detection of type A influenza virus and differentiation of avian H7 subtype

a reverse transcription-pcr assay for detection of type a influenza virus and differentiation of avian h7 subtype

abstract : avian influenza virus (aiv) infection is a major cause of influenza mortality in birds and can cause human mortality and morbidity. although the risk of infection with avian influenza virus (aiv) is generally low for most people, the pathogenic virus can cross the species barrier and acquires the ability to infect and be transmitted among the human population; therefore the rapid ide...

multiplex reverse transcription-pcr assay for detection of type a influenza virus plus differentiation of avian h7 and h9 hemagglutinin subtypes in iran

background : avian influenza virus (aiv) infection is a major cause of bird and human morbidity and mortality. we aimed to evaluate a specific and sensitive multiplex rt-pcr that can simultaneously detect influenza type a viruses and differenti­ate the two most important subtypes of avian influenza viruses h7 and h9 subtypes. methods : a multiplex reverse transcriptase-polymerase chain reaction...

Validation of a real-time reverse transcriptase-PCR assay for the detection of H7 avian influenza virus.

This report describes the validation of an avian influenza virus (AIV) H7 subtype-specific real-time reverse transcriptase-PCR (rRT-PCR) assay developed at the Southeast Poultry Research Laboratory (SEPRL) for the detection of H7 AI in North and South American wild aquatic birds and poultry. The validation was a collaborative effort by the SEPRL and the National Veterinary Services Laboratories...

Isolation and Identification of Avian Influenza Virus H9N2 Subtype

Multiplex Reverse Transcriptase-PCR Assay for Typing and Subtyping of Influenza A (H5 & H9) Virus in Iran

Avian influenza virus (AIV) infection is a major cause of bird or human mortality and morbidity, therefore the rapid identification of the virus is of important clinical and epidemiological implication. Methods: A multiplex Reverse Transcriptase PCR (RT-PCR) was optimized for the detection of influenza A virus and the H5 and H9 subtypes. The influenza type A specific primers were directed to t...

Detection of avian influenza virus of H9 subtype in the feces of experimentally infected chickens by RT–PCR

  Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...