بررسی نقش ژنهای JAK2 و STAT3 در سرطان ریه

Title: Evaluation of expression and methylation of promoter of JAK2 and STAT3 genes in lung cancer tissue and its comparison with adjacent healthy tissue in 75 people with this cancer.             Objective: Lung cancer is a disease that affects lung tissue cells. It is a common cancer in the world and is one of the deadliest cancers. Molecular variations of genes have a significant effect on the progression of the disease and blockage. These pathways can improve the treatment process. The purpose of this study was to evaluate the modifications of the promoter methylation of JAK2 / STAT3 signaling pathways and the level of expression of these genes at the level of mRNA, which has so far been studied in lung cancer in Iran. Not action. Materials and Methods: In this research, a case control study, 75 samples of parathyroid cancer were collected and tumor margins were selected as control samples. Samples were collected from Imam Ali hospital in Zahedan from April to December 1995. Initially, de-paraffinization was performed, and then DNA and RNA were extracted for further investigation  The promoter methylation of JAK2 and STAT3 genes was evaluated using Metelyation-specific polymerase chain reaction (MSP) technique by using 75 samples of paraffin and parietal lung cancer tissue and soft tissue at the tumor margins as tissue control samples. Took The expression analysis was performed on paraffin embedded tissue samples (22 of the cancerous and normal tissues) using PCR real time. findings: . The results of the study indicate that there is a risk of lung cancer between the status of the promoter's methylation of both JAK2 and STAT3 genes. [JAK2: OR = 7.15; 95% CI: 3.35-15.46, p = 0.001, STAT3: OR = 8.77, 95% CI: 4.16-18.49, p = 0.001]. Conclusion: According to our knowledge, the present study is the first to show the association between the promoter of hypermethylation of the JAK2 / STAT3 gene, the reduction of JAK2 expression and an increased risk of lung cancer.