بررسی تأثیر سمیت گیاه Kelussia odoratissima Mozaff رویش یافته در دو منطقه کوهرنگ و فریدونشهر، بر رده سلول سرطان سینه انسانی MCF7 و سلول‌های تک هسته‌ای خون محیطی انسانی

Background: Kelussia Odoratissima (Apiaceae family) is an herbaceous plant which traditionally used in Iran as antiache, anti inflammation and anticoughagents. This plant has potent antioxidant and anticancer effects. The present investigation was carried out to study cytotoxic activity of leaves, shoots, seeds, flowers and roots extracts of K.odoratissima collected from two different regions against MCF7 cell line and peripheral blood monolayer cell (PBMC). Materials and methods: The cells used in this research, MCF7 a breast cancer cell lines and normal lymphocytes were cultured in PRMI-1640 supplemented with 10% Fetal Bovine Serum (FBS), 100 U/ml penicillin and 100 &mug/ml streptomycin and 5mM/L glutamine. The cytotoxic effect of the crude methanol extract of this plant at different concentrations (10, 100, 500 and 1000 &mug/ml) were performed by using MTT assay. Results: The crude methanol extracts of different organs of K. odoratissima grown in korhang didn’t have any cytotoxic effect, but the flower, leave and seed extracts of K. odoratissima grown in fereydonshahr showed potent cytotoxic effect on the MCF7 cells. However these extracts at different concentrations were not cytotoxic on normal cells. Conclusion: In conclusion, the results demonstrated that different extracts K. odoratissima grown in fereydonshahr could be evaluated as an anticancer agent.