ارتباط واریانت‌های نادر rs16972194 و rs56124946 ژن TNFSF13B با پره‌اکلامپسی: یک مطالعه مورد- شاهدی

Background and Objectives: Preeclampsia is a pregnancy-related syndrome and the most common hypertensive disorder in pregnancy. Feto-maternal immune incompatibility, oxidative stress, genetic variants, and endothelial cells injuries play an important role in the pathogenesis of the disease. TNFSF13B gene, a member of tumor necrosis factor (TNF) family, is a main regulator of an immune response. This study was done with the aim of investigating the association between the rs16972194 and rs56124946 rare polymorphisms of TNFSF13B gene and preeclampsia disease. Materials and Methods: In this case-control study, 308 women with preeclampsia and 292 healthy pregnant women, referring to the reference clinic for women’s diseases in Hazrat Zeinab hospital in Shiraz from January to August 2016, were selected and underwent molecular testing. Genotyping of the rs16972194 and rs56124946 polymorphisms were determined using ARMS PCR and T-ARMS PCR, respectively. The data were analyzed using logistic regression and chi-square test. Results: The frequency of CC, CG, and GG rs56124946 polymorphism genotypes in the patients were respectively 95.2, 3.1, and 1.7 percent and 97.7, 1.3 and 1 percent in the controls. Statistically significant difference was not found in the frequency of genotypes and alleles of this polymorphic site between the case and control groups (p> 0.05). A significant percent of the controls (99.3%) and patients (99.7%) showed GG genotype for this polymorphism. No significant difference in the genotype frequencies was observed between the cases and controls (p> 0.05). Conclusion: The results showed that the rare variants of the TNFSF13B gene are not probably related to preeclampsia disease. Key words: Preeclampsia, Genetic variant, TNFSF13B gene, Iran   Funding: This research was funded by Islamic Azad University, Arsanjan Branch. Conflict of interest: None declared. Ethical approval: The Research Committee of Islamic Azad University, Arsanjan Branch approved the study (16030503942014).    How to cite this article: Bostany S, Nasiri M. Association of rs16972194 and rs56124946 Rare Variants of TNFSF13B Gene with Preeclampsia in Women Referring to Hazrat Zeinab Hospital in Shiraz in 2016. J Rafsanjan Univ Med Sci 2018; 17 (6): 539-52. [Farsi]