Use of Mesenchymal Adult Stem Cell for Cartilage Regeneration by Hydrogel

Authors

  • Farzaneh Tafvizi Department of Biology, Islamic Azad University, Parand Branch, Parand, Iran.
  • Gholamabbas Nasr Eurasia University of Science and Research, International Campus, Tehran, Iran
  • Mahdieh Ghiasi Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.
  • Mohammad Mehdizadeh Dental and Oral Research Center, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Qom University of Medical Sciences, Qom, Iran
  • Neda Tekiyeh Maroof Department of Molecular Biology, Islamic Azad Medical University of Tehran, Tehran, Iran.
  • Sahar Farzaneh Department of Biology, Islamic Azad University, Parand Branch, Parand, Iran.
  • Sedigheh Safari Department of Biology, Islamic Azad University, Tehran Science and Research Branch, Tehran, Iran .
Abstract:

Background and Aims: Cartilage is a very specific tissue, which does not have the capacity to heal and renew itself. Although the invention of the method of surgery with autologous chondrocyte transplantation, developed tools to treat the cartilage lesions, it couldn’t gain a great success due to problems such as damage to the area of donation. Using the mesenchymal stem cells derived from adipose and culturing and differentiating them on scaffolds was considered appropriate as a successful research and clinical strategy. Materials and Methods: In the present study, the mesenchymal stem cells were separated from adipose tissue and cultured in two scaffolds of fibrin glue and alginate medium. After 1, 7 and 14 days of cell differentiation, the survival ability of the differentiated cells were analyzed by Chondrogenic MTT. Moreover, type I and II collagen, aggrecan and Sox9 expression were measured via real time-polymerase chain reaction. In addition, cartilage reconstruction on scaffolds was shown by a histological investigation. Results: Our results showed that the expression of CD90 and CD105 as mesenchymal markers is at a high level whereas the expression of CD34 and CD45 reaches a low level. The LSD test demonstrated that there was no remarkable difference among the chondrogenic MTT, scaffolds groups and control in 7 and 14 days after cell differentiation (p<0.05), although, fibrin glue had the highest expression in chondrogenic gens. Conclusions: Finding suggests that in order to utilize a new strategy for tissue regeneration utilization of inherent scaffolds such as fibrin glue can act as a protector for mesenchymal stem cells.

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Journal title

volume 6  issue 3

pages  207- 218

publication date 2019-08

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