Transplantation of Cardiogenic Pre-Differentiated Autologous Adipose-Derived Mesenchymal Stem Cells Induced by Mechanical Loading Improves Cardiac Function Following Acute Myocardial Infarction in Rabbit Model

Authors

  • Baharak Emami National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
  • Elena Mahmoudi Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Mir Sepehr Pedram Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Mohammad Mehdi Dehghan Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Mohammad Molazem Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Saeed Farzad Mohajeri Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Suzan Amin Cardiovascular Lab, Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
  • Yasamin Valy Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Abstract:

Objective- Investigate myocardial performance after autologous adipose-derived (ASCs) mesenchymal stem cell differentiated under equiaxial cyclic strain, transplantation in rabbits with acute myocardial infarction (AMI). Design- Prospective, randomized experimental study Animals- 20 New Zealand White rabbits (2-3 kg) Procedure- ASCs were studied in four distinct groups of mechanical (ADM), chemical (ADC), undifferentiated (AD) and control (C) groups. According to this categorization, the cells were exposed to cyclic mechanical loading or 5-azacytidine as the chemical factor. 10 6 ASC cells were transplanted intramyocardially in rabbits with AMI (Acute Myocardial Infarction). Echocardiographic study was used to evaluate effects of cells on cardiac function. Results- Left ventricle ejection fraction (LVEF) was significantly increased in the ADM (mechanically-differentiated adipose-derived mesenchymal stem cell) group at 2 months follow-up. Fractional shortening (FS) also showed a similar pattern as LVEF and increased in ADM group in compare to control and AD (undifferentiated adipose-derived mesenchymal stem cell) group. Conclusion and clinical prevalence- The results indicate that intramyocardial transplantation of mechanically-differentiated ASCs improves cardiac function of ischemic myocardium. Transplantation of mechanically-differentiated ASCs for myocardial regeneration may become the future therapy for acute myocardial infarction.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Effects of mesenchymal stem cells with injectable scaffold on cardiac function in myocardial infarction in Rabbit

BACKGROUND: Bone marrow-derived mesenchymal cellscan transdifferentiate into Cardiomyocyte cells and improveheart function after transplantation. Since biomaterials canimprove the cell retention in the site, cell survival and differentiation,heart tissue engineering is now being explored as anapplied solution to support cell-based therapies and increasetheir efficacy for myocardial diseases. Ch...

full text

Repair of Old Myocardial Infarction by Intracoronary Transplantation of Autologous Bone Marrow Mesenchymal Stem Cells: A Pilot Clinical Trial

Experimental and clinical studies have shown that intracoronary transplantation of autologous bone marrow mesenchymal stem cells (BMSCs) has resulted in regenerated infarcted myocardium and improved left ventricular (LV) function. <span style="font-variant: normal; font-style: normal; f...

full text

Spermatogenesis after transplantation of adipose tissue-derived mesenchymal stem cells in busulfan-induced azoospermic hamster

Objective(s): Adipose tissue-derived mesenchymal stem cells (AT-MSCs) with more potent immunomodulatory effects, greater proliferative potential and secretion of growth factors and cytokines in comparison with bone marrow derived MSCs are more appropriate for cell therapy. The aims of the present study were to evaluate the histomorphometric effect of AT-MSCs allotransplantation on regeneration ...

full text

effects of mesenchymal stem cells with injectable scaffold on cardiac function in myocardial infarction in rabbit

background: bone marrow-derived mesenchymal cellscan transdifferentiate into cardiomyocyte cells and improveheart function after transplantation. since biomaterials canimprove the cell retention in the site, cell survival and differentiation,heart tissue engineering is now being explored as anapplied solution to support cell-based therapies and increasetheir efficacy for myocardial diseases. ch...

full text

Efficacy of Atorvastatin combined with adipose-derived mesenchymal stem cell transplantation on cardiac function in rats with acute myocardial infarction.

Mesenchymal stem cells (MSCs) have been extensively applied for the restoration of cardiomyocytes loss after acute myocardial infarction (AMI). However, the optimal therapeutic efficacy of MSCs in ischemic heart diseases has been hampered by their poor survival and low differentiated rates. Therefore, the improvement of MSC survival and differentiated rates is warranted and critical for the eff...

full text

Intracoronary administration of autologous adipose tissue-derived stem cells improves left ventricular function, perfusion, and remodelling after acute myocardial infarction.

AIMS This study was designed to assess whether intracoronary application of adipose tissue-derived stem cells (ADSCs) compared with bone marrow-derived stem cells (BMSCs) and control could improve cardiac function after 30 days in a porcine acute myocardial infarction/reperfusion model. METHODS AND RESULTS An acute transmural porcine myocardial infarction was induced by inflating an angioplas...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 11  issue 2

pages  21- 30

publication date 2017-05-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023