The Expression of Signal Regulatory Protein-alpha in Normal and Osteoarthritic Human Articular Cartilage and Its Involvement in Chondrocyte Mechano-transduction Response
Authors
Abstract:
Signal regulatory proteins (SIRP) belong to immunoglobulin super family (IgSF) and relate to integrin signaling cascades. It has been shown that SIRPa is expressed in a variety of cells including myeloid cells and neurons. In the present study the expression of this IgSF member in articular chondrocytes was investigated. Methods: Using a panel of anti-SIRPalpha antibodies, immunohistochemistry, Western-blotting and methods, expression of SIRP electrophysiologya and its role in chondrocyte mechano-transduction were assessed. Results: No identifiable positive signal was obtained by using immunohistochemistry methods on frozen and paraffin sections. SIRPa is expressed by both normal and osteoarthritis cultured chondrocytes. The electrophysiological response of chondrocytes in the presence of SE7C2 mAb was significantly inhibited whereas SE5A5 did not show any modification in this response. Conclusions: It seems likely that SIRPa could be associated with other proteins such as integrins, CD47 and ion channels, which contribute to the electrophysiological response of human articular chondrocytes. In any case, this study has provided a specific functional role for SIRPalpha in chondrocyte mechano-transduction.
similar resources
the expression of signal regulatory protein-alpha in normal and osteoarthritic human articular cartilage and its involvement in chondrocyte mechano-transduction response
signal regulatory proteins (sirp) belong to immunoglobulin super family (igsf) and relate to integrin signaling cascades. it has been shown that sirpa is expressed in a variety of cells including myeloid cells and neurons. in the present study the expression of this igsf member in articular chondrocytes was investigated. methods: using a panel of anti-sirpalpha antibodies, immunohistochemistry,...
full textThe expression of signal regulatory protein-alpha in normal and osteoarthritic human articular cartilage and its involvement in chondrocyte mechano-transduction response.
BACKGROUND Signal regulatory proteins (SIRP) belong to immunoglobulin super family (IgSF) and relate to integrin signaling cascades. It has been shown that SIRPalpha is expressed in a variety of cells including myeloid cells and neurons. In the present study the expression of this IgSF member in articular chondrocytes was investigated. METHODS Using a panel of anti-SIRPalpha antibodies, immun...
full textDifferential Immunohistochemical Expression Pattern of Galectin-3 in Normal and Osteoarthritic Human Articular Cartilage
Background: Previous studies have shown that Galectin-3, a member of lectin family, is expressed in developing cartilage in mouse embryo and also in growth plate of long bones. Objective: In the present work, the expression pattern of Galectin-3 in normal and various grades of osteoarthritic (OA) human articular cartilage has been studied. Methods: Using immunohistochemistry and standard we...
full textEvaluation of CD98 Expression in Normal and Osteoarthritic Human Articular Chondrocytes
Background: Recent studies have provided evidence that integrins play roles in recognition of mechanical stimuli and its translation into a cellular response. Integrin signaling may be regulated by a number of mechanisms including accessory proteins such as CD98 (4F2 antigen). Objectives: To determine CD98 expression by human articular chondrocytes and its involvement in human articular mechano...
full textdifferential immunohistochemical expression pattern of galectin-3 in normal and osteoarthritic human articular cartilage
background: previous studies have shown that galectin-3, a member of lectin family, is expressed in developing cartilage in mouse embryo and also in growth plate of long bones. objective: in the present work, the expression pattern of galectin-3 in normal and various grades of osteoarthritic (oa) human articular cartilage has been studied. methods: using immunohistochemistry and standard we...
full textMy Resources
Journal title
volume 11 issue 2
pages 119- 124
publication date 2007-04
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023