The effects of pre and post vitrification taxol treatment on bovine immature oocyte

The aim of the present study was to find the effects of pre and post vitrification taxol treatment on bovineimmature cumulus oocyte complexes (COCs). Bovine ovaries were collected from the local slaughterhousein a container with 30-37°C physiologic saline and supplemented with antibiotics. The grade A aspiratedCOCs were assigned to 6 experimental groups; The 1st group was control (n=100) in which the COCs weresubjected to in vitro maturation (IVM) in oocyte maturation medium (OMM). The 2nd group was the COCs(n=51) that were exposed to vitrification and warming solutions and then subjected to IVM. The 3rd groupwas the COCs which were incubated in OMM contained taxol (1 μM, n=51) for 20 min then transferred toIVM. The 4th group was the COCs (n=50) that were vitrified, warmed and transferred to IVM. The 5th group(n=57) was the COCs which were incubated in OMM containing taxol (1 μM for 20 min), vitrified (solutionswith 1 μM taxol), warmed and transferred to IVM. The last group was the COCs (n=58) that were vitrifiedand warmed, then were incubated in maturation medium containing taxol (1 μM for 20) and, finally, weretransferred to IVM. The COCs of all groups were incubated in OMM for 24 h under a humified (95%)condition with a temperature of 39°C and 5% CO2. Oocyte maturation was not affected by exposing theCOCs to vitrification solution or taxol groups compared to control (P>0.05). Vitrification reduced the oocytematuration rate compared to control (P