The Effect of Threonine and Vitamin A on Immune System in Broiler Chickens

Two independent experiments were conducted with broilers to determine the effect of threonine and vitamin A on immune system. In first experiment, dietary treatments contained 0.8%, 0.87%, 0.94 % and 1.01 % total threonine. In second experiment, diets were as follows: basal diet without vitamin A; basal diet plus 1500 IU/Kg vitamin A; basal diet plus 6250 IU/Kg vitamin A; basal diet plus 11000 IU/Kg vitamin A. Fourteen days after feeding the treatments, cutaneous basophil hypersensitivity, cell-mediated immune response, was determined as the increase in toe-web skin thickness after an injection with phytohemagglutinin-P and twenty-two days after feeding the diets, heterophils and lymphocytes were enumerated. Humoral immunity was assessed by intravenous injection of 7% sheep red blood cell at 22 days of age. In first experiment, the titers of immunoglobulin for responses were numerically, but not significantly, increased in birds fed diet containing 0.87 % threonine and decreased in 0.94% and 1.01% threonine. Cutaneous basophil hypersensitivity was not affected in chicks, whereas there was a tendency for an increase in birds fed a diet containing 0.87 % threonine at 24 h after injection. In second experiment, the titers of immunoglobulin for responses were significantly increased in birds fed diet containing vitamin A. Cutaneous basophil hypersensitivity was also increased by addition vitamin A in the diet. The results obtained on the present study indicated that threonine and Vitamin A requirements of broiler based on recommendation of national research council are not sufficient to meet the requirement of the new commercial poultry