TaqMan real-time PCR: a reliable method to detect meat species

Authors

  • Mahyar Sharifan Central Laboratory of Veterinary Head Office of North Khorasan Province, Iran
  • Maryam Azizkhani Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
  • Maryam Torabi Central Laboratory of Veterinary Head Office of Khorasan Razavi Province, Iran
  • Sahar Porzahmat Shirvan Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
Abstract:

The aim of this study was to detect and identify meat species (beef, lamb, pork, chicken, donkey, and horse) in Kebab as a Halal meat-based food sold in on-road-restaurants of North Khorasan province (Iran) using TaqMan real-time polymerase chain reaction (PCR). Raw Kebab samples (150 samples) were obtained from on-road restaurants. The samples were prepared, DNA was extracted and TaqMan real-time PCR using beef, lamb, pork, chicken, horse, and donkey target genes was performed. The results indicated that 100% of samples yielded positive results of beef, but none of them generated donkey and pork amplicons. The samples of Kebab indicated 93.33% lamb, 83.33% chicken, 10% horse positive results. The information claimed for the source of meat was not correct for more than 95% of the samples and only one food center labeled the Kebab mix correctly. Except for one Kebab sample that showed a positive result for the presence of horse meat, no sample contained meat of species considered illegal in Halal foods.

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Journal title

volume 3  issue 2

pages  116- 120

publication date 2020-12-01

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