Tachykinin stimulates α-amylase, protease and lipase activities in the midgut of the American cockroach, Periplaneta americana (Blattodea: Blattidae)

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Abstract:

       در این تحقیق به بررسی اثر تاکیکینین بر روی فعالیت آلفا آمیلاز، پروتئاز و لیپاز در معده میانی سوسری امریکایی (L.) Periplaneta americana پرداخته شد. فعالیت ایمنوهیستوشیمیایی سلول‌های معده میانی (بخش های جلویی، میانی و عقبی) که تاکیکینین ترشح می‌کردند مورد تایید قرار گرفت. چهار هفته گرسنگی، تعداد سلول هایی را که عکس العمل ایمنوهیستوشیمیایی مقابل آنتی‌بادی تاکیکینین داشتند (Tachykinin-ir cells) را کاهش داد، در حالی که تغذیه دوباره پس از سه ساعت Tachykinin-ir cells را افزایش داد. آزمون الایزا نیز نتیجه فوق را تایید کرد. قرار دادن معده میانی در بافر حاوی تاکیکینین منجر به افزایش فعالیت آلفا آمیلاز، پروتئاز و لیپاز شد در حالی که بافر به تنهایی هیچ اثری بر روی فعالیت آنزیم‌های فوق نداشت. تزریق تاکیکینین به همولنف حشره منجر به افزایش فعالیت آنزیم‌های فوق در معده میانی شد، در حالی که تزریق بافر هیچ تاثیری بر روی فعالیت آنزیم ها نداشت. به طور کلی می‌توان نتیجه گرفت که تغذیه باعث ترشح تاکیکینین از سلول های معده میانی شده که آن نیز به نوبه خود فعالیت آنزیم های آلفا آمیلاز، پروتئاز و لیپاز را افزایش می‌دهد. 

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Journal title

volume 36  issue 2

pages  81- 88

publication date 2016-08-22

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