Synthesis a New Viral Base Vector Carrying Single Guide RNA (sgRNA) and Green Florescent Protein (GFP)

pHluorin2: an enhanced, ratiometric, pH-sensitive green florescent protein.

Green florescent protein (GFP) variants that are sensitive to changes in pH are invaluable reagents for the analysis of protein dynamics associated with both endo- and exocytotic vesicular trafficking. Ratiometric pHluorin is a GFP variant that displays a bimodal excitation spectrum with peaks at 395 and 475 nm and an emission maximum at 509 nm. Upon acidification, pHluorin excitation at 395 nm...

“تولید انگل leishmania major تراریخته بیان کننده ژن green fluorescent protein (gfp)، به کمک ادغام ژن gfp به درون پروموتر rna ریبوزومی انگل“

تاکنون 6 گونه لیشمانیا شناخته شده است که باعث بیماری لیشمانیوز در انسان می شوند. از بین آن لیشمانیا اینفانتوم باعث بیماری کشنده کالا آزار و لیشمانیا ماژور باعث عوارض پوستی با ایجاد اسکار میشود. یکی از دلایل اهمیت کار روی لیشمانیا، شیوع بالای این بیماری است. در بررسی های تحقیقاتی که بر روی مدلهای حیوانی صورت گرفته است چندین روش استاندارد و در عین حال دشوار و وقتگیر (نظیر مشاهده فرم آماستیگوت انگ...

Discovery of Green Fluorescent Protein, Gfp

I discovered the green fluorescent protein GFP from the jellyfish Aequorea aequorea in 1961 as a byproduct of the Ca-sensitive photoprotein aequorin (Shimomura et al., 1962; Johnson et al., 1962), and identified its chromophore in 1979 (Shimomura, 1979). GFP was a beautiful protein but it remained useless for the next 30 years after the discovery. My story begins in 1945, the year the city of N...

Endogenous green fluorescent protein (GFP) in amphioxus.

Improved silencing vector co-expressing GFP and small hairpin RNA.

Small interfering RNA (siRNA) is a powerful tool for the specific silencing of gene expression. We developed an improved vector, pG-SUPER, that co-expresses green fluorescent protein (GFP) and small hairpin RNA simultaneously to facilitate analysis of silencing at the level of individual cells. As a test system, we analyzed lamin A/C knockdown in HeLa cells. The GFP signal was a reliable report...

Introduction of green fluorescent protein (GFP) into hippocampal neurons through viral infection.

Expression of green fluorescent protein (GFP), its more fluorescent mutant forms (e.g., EGFP [enhanced GFP]), or their fusion protein derivatives, affords a number of informative possibilities in cellular neuroscience. EGFP is a soluble protein and appears to be homogeneously distributed within the cytosol of neurons when expressed. Thus, it reveals the structure of the neuron, including the ce...