Successful cryopreservation of in vitro derived bovine blastocysts in microcapillary pipette tips
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Abstract:
The open pulled straw has been used effectively to vitrify preimplantation embryos because of geometricfeatures that allow rapid rates of temperature exchange. One possible inexpensive alternative to the openpulled straw are commercially-available microcapillary pipette tips commonly used for electrophoresis. Themain purpose of this study was to compare the survival rates of in vitro produced blastocysts followingvitrification in microcapillary pipette tips and open pulled straws. Two experiments were conducted toinvestigate the effect of type of carrier, age of expanded blastocyst, and addition of β-mercaptoethanol topost-warming culture medium on survival of vitrified in-vitro derived blastocysts. Expanded blastocysts (Day7, 0900H and 1900H after insemination; insemination = Day 0 at 0900H) were vitrified while loadedrandomly in groups of 4-10 into open pulled straws or pipette tips. Following warming, embryos werecultured in groups of up to 20 in 20 μl microdrops of modified KSOM or modified KSOM containing 100μM β-mercaptoethanol at 38.5°C in 5% CO2 in air. Survival after warming was assessed as the percentage ofvitrified embryos that re-expanded and the percent that hatched after 48 hrs culture. Post-warming survivalrates were not affected by type of carrier or age of the expanded blastocyst (P>0.05). The proportions ofembryos that re-expanded (55.5%) and hatched (25.7%) were higher (P
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Journal title
volume 8 issue 1
pages 1- 7
publication date 2007-03-30
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