Study of Kell blood group genotype in alloimmiunized thalassemia patients

Authors

Abstract:

Abstract Background and Objectives Alloimmunization is the most serious problem in thalassemia patients and Anti-K is the most prevalent antibody in these patients. So accurate identification of this antigen can significantly decrease the rate of alloimmunization. Serological phenotyping is usually not reliable in multi-transfused patients. Molecular genotyping can overcome limitations of hemagglutination assays, resolve discrepant serologic typing and guide RBC selection for them. In this regard, we intended to determine the K and k among alloimmunized thalassemia patients using molecular methods and compare the results between different methods.    Materials and Methods In this descriptive study, a total of 200 blood samples were collected randomly from alloimmunized thalassemia patients of Tehran Adult Thalassemia Clinic. The phenotype of all samples was determined for K and k. PCR-SSP was performed for all samples. The discrepant results between the phenotype and genotype were re-evaluated by PCR-RFLP and were confirmed by DNA sequencing.   Results Sixty-three (28%) out of 200 patients developed Anti-K. Molecular typing of samples for K and k antigens revealed 96% (192 patients) KEL*02/KEL*02 and 4% (8 patients) KEL*01/KEL*02 among our samples. Discrepancy between the serology and genotyping results were detected in 8 cases that in all cases correction of genotype results was confirmed by DNA sequencing.   Conclusions  This study demonstrates that antibodies against K antigen continue to develop in thalassemia patients at high rate. Our findings suggest that RBC molecular genotyping is superior to serological phenotyping and is a good alternative and more reliable method especially in multi transfusion patients such as thalassemia patients.  

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

The prevalence of undesired blood group antibodies in thalassemia patients

Abstract Background and Objectives Blood transfusion related alloimmunization is common among patients receiving regular blood transfusion, especially thalassemia patients. In this study we aimed to find the prevalence of alloimmunization among patients of thalassemia.   Materials and Methods In this desciptional study, we screened 305 thalassemia patients (53.8% female and  46.2% male, age ...

full text

Expression of Kell blood group protein in nonerythroid tissues.

The Kell blood group protein is a zinc endopeptidase that yields endothelin-3, a potent bioactive peptide, by cleavage of big endothelin-3, a larger intermediate precursor. On red cells, Kell protein is linked by a single disulfide bond to XK, a protein that traverses the membrane 10 times and whose absence, as occurs in the McLeod phenotype, is associated with a set of clinical symptoms that i...

full text

Inactivation of Kell blood group antigens by 2-aminoethylisothiouronium bromide.

Human red cells incubated with a solution containing 6% 2-aminoethylisothiouronium bromide (AET) lose activity of antigens that are part of, or related to, the Kell blood group system. However, Kx antigen is not inactivated. Studies on a wide range of other blood-group antigens show no other evidence of changes and AET appears to react specifically with red-cell membrane structures that have Ke...

full text

A STUDY OF THE INTRACELLULAR KILLING OF THE PERIPHERAL BLOOD NEUTROPHILS IN β- THALASSEMIA MAJOR PATIENTS

52 β- thalassemia major patients were studied. Their ages ranged from two to 20 years. The group consisted of 22 girls and 30 boys. Candida intracellular killing and NBT (nitroblue tetrazolium) tests were performed on the peripheral blood neutrophils of these patients. Average results of the intracellular killing test was within normal range for the patients, but showed an appreciable decr...

full text

A study of peripheral blood neutrophils’ function in patients suffering from thalassemia major

Background: Thalassemia is the most common blood disorder in the world. This hereditary anemia, Which develops due to a constitutional imbalance in alpha and beta chains of adult haemoglobin (hbA), has serious and life- threatening complications (accompanied by haemolysis and bone deformities) in its major form. We may encounter a diverse collection of articles in world research centers about t...

full text

Prevalence of Rh, Duffy, Kell, Kidd & MNSs blood group antigens in the Indian blood donor population

BACKGROUND & OBJECTIVES Little data are available regarding the frequencies of the blood group antigens other than ABO and RhD in the Indian population. Knowledge of the antigen frequencies is important to assess risk of antibody formation and to guide the probability of finding antigen-negative donor blood, which is especially useful when blood is required for a patient who has multiple red ce...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 16  issue 4

pages  259- 269

publication date 2019-12

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023