Short communication:In vitro screening of Sonneratia alba extract against the oomycete fish pathogen, Aphanomyces invadans
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Immunofluorescence of the epizootic ulcerative syndrome pathogen, Aphanomyces invadans, using a monoclonal antibody.
A monoclonal antibody (MAb), designated 3gJC9, was raised against a protein antigen of Aphanomyces invadans, the oomycete pathogen that causes epizootic ulcerative syndrome (EUS). The antigen was expressed on the surface of hyphae and secreted extracellularly. MAb 3gJC9 did not cross-react with other oomycete or fungal pathogens of fish, although it did react to the crayfish plague pathogen A. ...
full textInfection experiments with Aphanomyces invadans in four species of estuarine fish.
Along the eastern seaboard of the US, Atlantic menhaden, Brevoortia tyrannus, develop characteristic ulcerative lesions, a condition termed ulcerative mycosis. These lesions are identical to those seen across Asia in fish affected by epizootic ulcerative syndrome, a condition caused by the fungus-like oomycete Aphanomyces invadans. Young-of-the-year menhaden inhabiting estuarine environments ar...
full textMonoclonal antibody (MAb) based immunodot for early detection of Aphanomyces invadans in fish
untius sp were injected with 0.1ml spores of Aphanomyces invadans intramuscularly into the left dorsal trunk muscle. Fish were collected at an interval of 1, 3, 5, 10, 24, 48h and 4d after the injection. The muscle tissue was removed from the injection site, away from the injection site, far away from the injection site and other side of the injection site and used for MAb based immunodot and h...
full textMolecular assays for detecting Aphanomyces invadans in ulcerative mycotic fish lesions.
The pathogenic oomycete Aphanomyces invadans is the primary etiological agent in ulcerative mycosis, an ulcerative skin disease caused by a fungus-like agent of wild and cultured fish. We developed sensitive PCR and fluorescent peptide nucleic acid in situ hybridization (FISH) assays to detect A. invadans. Laboratory-challenged killifish (Fundulus heteroclitus) were first tested to optimize and...
full textCharacterization of Aphanomyces invadans by electrophoretic and Western blot analysis
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Journal title
volume 16 issue None
pages 1333- 1340
publication date 2017-10
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