Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

separation of somatropin and its degradation products by high-performance liquid chromatography using a reversed-phase polymeric column

the accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. in this study, a gradient rp-hplc method is described for the separation of human growth hormone (hgh) variants as deamidated and oxidized forms. the methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1ml/min flow rate of a linear gradient of 0.1% v/...

Reversed-phase high-performance liquid chromatography.

Separation of a complex mixture of heparin-derived oligosaccharides using reversed-phase high-performance liquid chromatography.

A reversed-phase ion-pairing high-performance liquid chromatography (RP-HPIPC) method for the separation of a complex mixture of heparin-derived oligosacchrides has been developed by a stepwise optimization of the mobile phase, in which the concentration of ion-pairing reagent, mobile phase pH, and acetonitrile concentration were varied. The resolution of more than 30 oligosaccharide components...

Separation of actinomycins and biosynthetic analogues by normal and reversed-phase high performance liquid chromatography.

Chromatographic procedures for the separation and identification of actinomycins have been reviewed1}. Only one report of HPLC has appeared2); this described reversed-phase separations of the C complex and of a biosynthetic mixture of actinomycin D (AMD)and congeners containing c/s-4-chloroproline in place of one or both prolines. In the present study a wider range of naturally occurring actino...