Royal Jelly Promotes Ovarian Follicles Growth and Increases Steroid Hormones in Immature Rats

Objective Royal jelly (RJ) is a complementary diet widely used by traditional healers for the treatment of infertility. The aim of present study was to evaluate the effect of RJ on some reproductive parameters in immature female rats. MaterialsAndMethods In this experimental study, thirty two immature female rats (30-35gr) were divided into four groups (n=8 /group). Control group received 0.5 ml distilled water interaperitonealy (i.p) and experimental groups received 100, 200 and 400 mg/kg/body weight doses of RJ daily for 14 days. Animals were sacrificed and ovaries were dissected for histologic examination. The serum levels of ovarian hormones, nitric oxide (NO) and ferric reducing antioxidant power (FRAP) were evaluated. The ratio of the ovarian and uterine weight to body weight was calculated. One-way ANOVA was used for data analysis. Results Results: The body weight showed a significant difference (P=0.002) between groups and was increased in all RJ treated animals. Uterine and ovarian weight and the serum levels of progesterone (P=0.013) and estradiol (P=0.004) were increased in experimental groups significantly. In addition, a significant increase in the number of mature follicles and corpora lutea (P=0.007) were seen. A significant increase in the serum levels of FRAP (P=0.009) and a significant decrease in NO level (P=0.013) were observed. Conclusion Royal jelly promotes folliculogensis and increases ovarian hormones. It can be considered a natural growth stimulator for immature female rats.