Retrospective Detection of Avibacterium Paragallinarum Serovar B in Egg Yolk Materials by PCR
Authors
Abstract:
Avibacterium paragallinarum is Gram-negative bacteria that cause infectious coryza (IC), an acute respiratory disease of chickens. Despite vaccination of Layer and breeder farms of Iran against IC, they are still experiencing the disease clinically and there is no knowledge of serotypes prevalence of this bacterium in this country. This study designed to determine serovar identity by molecular identification tests of previously studied isolates from Iran that were inactivated accidentally in egg yolk storing culture media. Genomes of the isolates extracted from egg yolk and then were subjected to two set of PCR tests using by published group specific and Page's serovar specific primers. The later primers were based on sequence differences in hypervariable region of haemagglutinin protein of Av. paragallinarum. These two sequential PCR confirmed that the bacteria were Av. paragallinarum and were associated to Page's serotype B. This is the first report of molecular detection of serotype B of Av. paragallinarum from Iran.
similar resources
retrospective detection of avibacterium paragallinarum serovar b in egg yolk materials by pcr
avibacterium paragallinarum is gram-negative bacteria that cause infectious coryza (ic), an acute respiratory disease of chickens. despite vaccination of layer and breeder farms of iran against ic, they are still experiencing the disease clinically and there is no knowledge of serotypes prevalence of this bacterium in this country. this study designed to determine serovar identity by molecular ...
full textAvibacterium paragallinarum pdf
Background: Avibacterium paragallinarum, the causative agent of infectious. Discussion and conclusion: The availability of A. paragallinarum.Abstract. Avibacterium paragallinarum serovar B was diagnosed and revealed in a layer farm.
full textValidation of Reference Genes for Real-Time Quantitative PCR (qPCR) Analysis of Avibacterium paragallinarum
Real-time quantitative reverse transcription PCR (qRT-PCR) offers a robust method for measurement of gene expression levels. Selection of reliable reference gene(s) for gene expression study is conducive to reduce variations derived from different amounts of RNA and cDNA, the efficiency of the reverse transcriptase or polymerase enzymes. Until now reference genes identified for other members of...
full textImmunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA.
Inactivated vaccines of Avibacterium paragallinarum provide protection and reduce the economic losses caused by infectious coryza. However, inactivated bacterins provide protection only against the Page serovars included in the vaccine. In this study, we investigated the immunological properties of a functional recombinant haemagglutinin protein (rHagA) derived from a Taiwan isolate strain A9 a...
full textDraft Genome Sequence of Avibacterium paragallinarum Strain 221
Avibacterium paragallinarum is the causative agent of infectious coryza. Here we report the draft genome sequence of reference strain 221 of A. paragallinarum serovar A. The genome is composed of 135 contigs for 2,685,568 bp with a 41% G+C content.
full textMy Resources
Journal title
volume 69 issue 2
pages 179- 183
publication date 2014-12-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023