Regulatory T Cell Subtypes and TGF-β1 Gene Expression in Chronic Allograft Dysfunction

Authors

  • Abtin Shahlaee Molecular Immunology Research Center, Tehran University of Medical Sciences
  • Adel Sepanjnia Department of Immunology, School of Medicine
  • Aliakbar Amirzargar Department of Immunology, School of Medicine | Molecular Immunology Research Center, Tehran University of Medical Sciences
  • Fateme Pourrezagholi Chronic Kidney Disease Research Center, Labbafinejad Hospital, Shahid Beheshti University of Medical Sciences
  • Mahboob Lessan Pezeshki Nephrology Research Center, Tehran University of Medical Sciences
  • Mahmoud Parvin Department of Pathology, Labbafinejad Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Mohammad Hossein Nicknam Department of Immunology, School of Medicine | Molecular Immunology Research Center, Tehran University of Medical Sciences
  • Mohsen Nafar Chronic Kidney Disease Research Center, Labbafinejad Hospital, Shahid Beheshti University of Medical Sciences
  • Pedram Ahmadpoor Chronic Kidney Disease Research Center, Labbafinejad Hospital, Shahid Beheshti University of Medical Sciences
  • Sara Assadiasl Department of Immunology, School of Medicine | Molecular Immunology Research Center, Tehran University of Medical Sciences
Abstract:

Background: Regulatory T cells have been suggested to have a protective role against acute rejection in allograft recipients. However, there is little information available about their contribution to chronic rejection process. The role of transforming growth factor-beta 1 (TGF- β1) as a profibrogenic and/or immunoregulatory cytokine in renal allografts is also controversial. Objectives: To evaluate the frequency of CD4+CD25+CD127- and CD3+CD8+CD28- regulatory T cells in chronic allograft dysfunction (CAD) and to investigate the expression of TGF- β1 in renal allografts. Methods: Thirty biopsy-proven CAD patients were pair-matched with 30 stable graft function patients and a third group of healthy volunteers. Flowcytometry was performed on PBMCs to determine the frequency of CD3+CD8+CD28- and CD4+CD25+CD127- regulatory T cells in lymphocyt population. TGF- β1 gene expression was assessed by Real Time PCR. Results: The percentage of CD3+CD8+CD28- Tregs among renal allograft recipients was higher than healthy controls (p

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Journal title

volume 11  issue 3

pages  139- 152

publication date 2014-09-01

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