Reduction of NADH oxidase, NO synthase, TNFα, and IL-1β mRNA expression levels on lipopolysacharide-stimulated murine macrophages by Zataria Multiflora
Authors
Abstract:
Zataria multiflora (ZM) is a thyme-like aromatic plant in the Lamiaceae family that grows in central and southern Iran. ZM is extensively used as a flavor ingredient in a wide variety of foods and is used as part of popular traditional folk remedies. In the present study, ZM essential oil (ZMO) was obtained from ZM leaves via hydro-distillation and then analyzed by GC-MS (gas chromatography-mass spectrometry). The anti-inflammatory activity of ZMO was determined via measures of NADH oxidase (NOX), inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, and interleukin (IL)-1β mRNA expression in lipopolysaccharide-stimulated murine macrophages using real-time polymerase chain reaction (PCR). GC-MS analysis indicated that the main components in the ZMO were carvacrol (29.4%), thymol (25.7%), p-cymene (11.2%), linalool (9.3%), and γ-terpinene (8.0%). ZMO significantly reduced NOX, iNOS, TNFα, and IL-1β mRNA expression in cells at concentrations of 0.1-1 μg/mL, indicating a capacity for this product to potentially modulate/diminish immune responses. ZMO has anti-oxidant and anti-inflammatory properties and could be potentially used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and a number of inflammatory conditions associated with stress.
similar resources
reduction of nadh oxidase, no synthase, tnfα, and il-1β mrna expression levels on lipopolysacharide-stimulated murine macrophages by zataria multiflora
zataria multiflora (zm) is a thyme-like aromatic plant in the lamiaceae family that grows in central and southern iran. zm is extensively used as a flavor ingredient in a wide variety of foods and is used as part of popular traditional folk remedies. in the present study, zm essential oil (zmo) was obtained from zm leaves via hydro-distillation and then analyzed by gc-ms (gas chromatography-mas...
full textاثر اسانس آویشن شیرازی بر تولید یون سوپراکسید و نیتریک اکسید و بیان ژنهای NADH اکسیداز و نیتریک اکسید سنتاز در سلولهای ماکروفاژ
Objective: In this study, inhibitory effect of Zataria multiflora essential oil on superoxide and nitric oxide production and NADH oxidase (NOX) and nitric oxide synthase (NOS) expression was examined. Materials and Methods: Zataria essential oil was obtained by hydro-distillation and chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS). Superoxide and ni...
full textA novel carboline derivative inhibits nitric oxide formation in macrophages independent of effects on TNFα and IL-1β expression
full text
Oral Administration of Zataria multiflora Extract Decreases IL-17 Expression in Perennial Allergic Rhinitis
Background: Rhinitis, which occurs most commonly as allergic rhinitis and affects 20% of the world’s population, is a major health care burden causing significant morbidity. Considering the high prevalence of allergic rhinitis and anti-inflammatory effects of thyme, a favorite condiment, we performed a randomized clinical trial to determine whether thyme can relieve allergic rhinitis symp...
full textCatabolic cytokine expression in degenerate and herniated human intervertebral discs: IL-1β and TNFα expression profile
Low back pain is a common and debilitating disorder. Current evidence implicates intervertebral disc (IVD) degeneration and herniation as major causes, although the pathogenesis is poorly understood. While several cytokines have been implicated in the process of IVD degeneration and herniation, investigations have predominately focused on Interleukin 1 (IL-1) and tumor necrosis factor alpha (TN...
full textThe Effect of MS14 on Production of Pro-inflammatory Cytokines by Macrophages
Objective(s) Using herbal medicines as a complementary treatment method is increasing in wide variety of diseases. MS14-an herbal-marine preparation-is reported to have anti-inflammatory and immunomodulatory activities; however, the mechanism underlying its therapeutic effect is not known. Macrophages play an important role in host defense mechanisms and carry out their role by producing vario...
full textMy Resources
Journal title
volume 2 issue 3
pages 89- 100
publication date 2013-07-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023