Rapid repair of skin ulcers in diabetic rats using polycaproplactone /gelatin nanon-fiber scaffold

Introduction: Three-dimensional nano scaffolds by creating a three-dimensional structure similar to the body tissues have created a new perspective on cell culture and tissue repair. The aim of this study was to investigate the effect of polycaprolactone biodegradation scaffold (PCL) in treatment of diabetic wounds. Materials and Methods: Nano PCL-Gelatine scaffold was synthesized by electrospun method. A circular wound (6 mm) was created in skin of 15 BALB/c rats and the rats were divided into 3 groups including: Non-diabetic group (sham), Diabetic group without nano scaffold receiving (control) and diabetic group with nano scaffolds (scaffold). Wound surface area and wound healing were evaluated on the 7th and 14th days using H & E staining with Image J and SPSS softwares. Results: On 7th day, the smallest and highest wound surface was observed in the Scaffold and Control groups respectively (P <0.04). On 14th day, the smallest and highest levels of ulcers were observed in sham and control groups respectively (P <0.00). In the evaluation of wound healing on 7th and 14th days, the highest treatment rates were observed in the Scaffold and sham groups respectively, and on the 14th day there was no significant difference between the two groups (0.40). Conclusion: Polycaprolactone nano-fiber scaffold has a good compatibility with skin ulcers in rats and it can increase the rate and extent of wound healing in diabetic rats.