Rapid molecular techniques for detection of foodborne Bacillus cereus pathogen
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Abstract:
Background and Objective: Bacillus cereus is responsible for several outbreaks of foodborne diseases. Therefore, the purpose of the study was to replace routine culture testing, by employing PCR directly from food to detect the bacteria and its enterotoxins. Methods: In the present study, a total of 75 Kibda sandwiches, Sausage sandwiches, Luncheon chicken, Luncheon meat and shawarma sandwiches (15 each) were collected from different places for bacteriological isolation, identification and rapid analysis by using PCR. Results: Bacterial isolation revealed 17 positive samples isolated from different food types, of those 17 samples, 40% were Kibda, 26.6% were Sausage, 20% was Luncheon chicken, 13.3% were positive for both Luncheon meat and shawarma sandwiches. Using PCR to identify B. cereus from positive isolates (group A) 8 isolates were detected with its enterotoxins genes nhe & cytK at 533, 766 and 421 bp respectively, Also the PCR, which used in detection of Bacillus cereus directly from food related to positive samples (group B) revealed that 8 Bacillus cereus were detected in food with its enterotoxins genes nhe & cytK, while group C which represents some random food samples of negative isolation result revealed that 3 Bacillus cereus positive strains were detected and its enterotoxins genes nhe & cytK. Conclusion: PCR assay was a sensitive & specific diagnostic tool in detection of Bacillus cereus with its enterotoxins genes directly from food samples, even in the presence of low numbers of B. cereus bacteria which cannot be detected by traditional isolation and identification
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Journal title
volume 17 issue 3
pages 12- 12
publication date 2023-06
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