RAPID IDENTIFICATION OF GROUP A STREPTOCOCCI BY PYRROLIDONYL-β-APHTHYLAMIDE HYDROLYSIS
Authors
Abstract:
In a clinical trial a new pyrrolidonyl-β-naphthylamide (PYR) hydrolysis test was compared with the bacitracin disk susceptibility test for accuracy in the presumptive identification of group A streptococci (GAS). Among 128 isolates of beta-hemolytic streptococci 93 group A isolates were found. The sensitivity of the PYR and bacitracin tests were similar (98.9%), but the bacitracin test had a lower specificity (80%) than the PYR test (100%). The efficiency of the PYR and bacitracin test were 99.2% and 93.7%, respectively. All bacitracin tests were performed on subcultures of the isolates from the primary plate, whereas PYR testing was performed on colonies from the primary plate. This shortened the turnaround time for the PYR test compared to the bacitracin test by at least 24 hours.
similar resources
rapid identification of group a streptococci by pyrrolidonyl-β-aphthylamide hydrolysis
in a clinical trial a new pyrrolidonyl-β-naphthylamide (pyr) hydrolysis test was compared with the bacitracin disk susceptibility test for accuracy in the presumptive identification of group a streptococci (gas). among 128 isolates of beta-hemolytic streptococci 93 group a isolates were found. the sensitivity of the pyr and bacitracin tests were similar (98.9%), but the bacitracin test had a lo...
full textRapid, cost-effective identification of group A streptococci and enterococci by pyrrolidonyl-beta-naphthylamide hydrolysis.
Group A streptococci and enterococci were identified by two L-pyrrolidonyl-beta-naphthylamide (PYR) hydrolysis tests. One uses Minitek PYR disks (BBL Microbiology Systems, Cockeysville, Md.); the other uses a swab impregnated with PYR reagent. Both tests and Strep-A-Chek (EY Laboratories, San Mateo, Calif.), a commercially available PYR test, were compared with conventional methods. The PYR met...
full textRapid identification of group B streptococci by counterimmunoelectrophoresis.
Beta-hemolytic streptococcal isolates have been examined by counterimmunoelectrophoresis (CIE) with group B antiserum to determine whether this techinque is of value in the rapid identification of group B strains. Ninety stock cultures and 100 clinical isolates of beta-hemolytic streptococci including representatives of groups A, D, C, G, and B were inoculated into Todd-Hewitt broth; after incu...
full textRapid hippurate hydrolysis method for presumptive identification of group B streptococci.
A rapid test to detect the hydrolysis of sodium hippurate by beta-hemolytic streptococci within 2 h was developed. All group B streptococci tested were positive using this method and all other groups were negative.
full textRapid diagnosis of pharyngitis caused by group A streptococci.
Although commercial rapid antigen detection tests (RADTs) are more expensive than blood agar plate (BAP) cultures, the advantage they offer is the speed with which they provide results. Rapid identification and consequent prompt treatment of patients with pharyngitis due to group A beta-hemolytic streptococci (GABHS) can reduce the risk of spread of GABHS, can allow patients to return to school...
full textIdentification of group A streptococci by direct fluorometry.
A simple direct fluorometric method for rapid identification of group A streptococci is described. The method permits the detection of the organism in mixed cultures without the aid of a microscope and is amenable to automated processing of specimens. Experience with the indirect fluorometric method revealed that nontrypsinized cells from a 10-fold dilution of overnight broth cultures could be ...
full textMy Resources
Journal title
volume 9 issue 3
pages 243- 245
publication date 1995-10
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023